Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QZL45_RS01740 Genome accession   NZ_CP129944
Coordinates   361967..362386 (-) Length   139 a.a.
NCBI ID   WP_222763982.1    Uniprot ID   -
Organism   Vibrio fluvialis strain I7A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356967..367386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZL45_RS01720 coaE 357477..358085 (-) 609 WP_222763974.1 dephospho-CoA kinase -
  QZL45_RS01725 pilD 358088..358957 (-) 870 WP_222763976.1 A24 family peptidase Machinery gene
  QZL45_RS01730 pilC 359009..360235 (-) 1227 WP_222763978.1 type II secretion system F family protein Machinery gene
  QZL45_RS01735 pilB 360271..361957 (-) 1687 Protein_327 type IV-A pilus assembly ATPase PilB -
  QZL45_RS01740 pilA 361967..362386 (-) 420 WP_222763982.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  QZL45_RS01745 nadC 362618..363505 (-) 888 WP_308413212.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QZL45_RS01750 ampD 363658..364209 (+) 552 WP_055452472.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QZL45_RS01755 fldB 364214..364735 (-) 522 WP_020430452.1 flavodoxin FldB -
  QZL45_RS01760 xerD 364876..365784 (+) 909 WP_222763985.1 site-specific tyrosine recombinase XerD -
  QZL45_RS01765 dsbC 365801..366565 (+) 765 WP_024374397.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14284.25 Da        Isoelectric Point: 8.4642

>NTDB_id=856603 QZL45_RS01740 WP_222763982.1 361967..362386(-) (pilA) [Vibrio fluvialis strain I7A]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVTKSELASGFATIKSVLTPAELYVQENGKISSGVSTITSLGV
SSAANSLGTLSISADNEITFSHTDGSAKGAKFVYTRTTGGWSCAYTAPAAVSAAAPKGC

Nucleotide


Download         Length: 420 bp        

>NTDB_id=856603 QZL45_RS01740 WP_222763982.1 361967..362386(-) (pilA) [Vibrio fluvialis strain I7A]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGGGTCCT
GTCGGCGATTGCCGTACCCGCTTATAAGGATTACGTAACCAAGAGTGAGTTAGCTTCTGGCTTTGCCACAATTAAATCCG
TGCTAACACCAGCAGAGTTATATGTCCAAGAAAATGGAAAGATTAGTAGTGGAGTAAGTACCATCACATCGCTGGGTGTT
TCCTCTGCCGCTAATAGCTTAGGCACACTAAGTATCTCAGCAGACAATGAAATCACCTTTTCTCACACTGATGGTTCAGC
AAAGGGCGCGAAGTTCGTATACACAAGAACAACTGGTGGCTGGTCATGTGCGTACACCGCCCCAGCAGCCGTGAGCGCAG
CAGCACCGAAAGGTTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

52.985

96.403

0.511
  pilA Acinetobacter baumannii strain A118

41.83

100

0.46
  pilA Pseudomonas aeruginosa PAK

40.667

100

0.439
  pilA Vibrio cholerae C6706

37.662

100

0.417
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.662

100

0.417
  pilA Vibrio cholerae strain A1552

37.662

100

0.417
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.152

94.964

0.381