Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V9Z40_RS02140 Genome accession   NZ_CP146667
Coordinates   457277..457954 (-) Length   225 a.a.
NCBI ID   WP_000178174.1    Uniprot ID   A0A0H2VBG7
Organism   Escherichia coli strain MS 16-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452277..462954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9Z40_RS02125 (V9Z40_02125) chiA 453635..456337 (+) 2703 WP_000773173.1 bifunctional chitinase/lysozyme -
  V9Z40_RS02130 (V9Z40_02130) bfd 456506..456700 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V9Z40_RS02135 (V9Z40_02135) bfr 456772..457248 (+) 477 WP_000675504.1 bacterioferritin -
  V9Z40_RS02140 (V9Z40_02140) pilD 457277..457954 (-) 678 WP_000178174.1 prepilin peptidase GspO Machinery gene
  V9Z40_RS02145 (V9Z40_02145) gspM 457954..458415 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  V9Z40_RS02150 (V9Z40_02150) gspL 458412..459575 (-) 1164 WP_001334232.1 type II secretion system protein GspL -
  V9Z40_RS02155 (V9Z40_02155) gspK 459590..460573 (-) 984 WP_001058506.1 type II secretion system minor pseudopilin GspK -
  V9Z40_RS02160 (V9Z40_02160) gspJ 460566..461153 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  V9Z40_RS02165 (V9Z40_02165) gspI 461146..461523 (-) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  V9Z40_RS02170 (V9Z40_02170) gspH 461520..462029 (-) 510 WP_001076036.1 type II secretion system minor pseudopilin GspH -
  V9Z40_RS02175 (V9Z40_02175) gspG 462037..462474 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24866.76 Da        Isoelectric Point: 7.6364

>NTDB_id=856601 V9Z40_RS02140 WP_000178174.1 457277..457954(-) (pilD) [Escherichia coli strain MS 16-3]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=856601 V9Z40_RS02140 WP_000178174.1 457277..457954(-) (pilD) [Escherichia coli strain MS 16-3]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VBG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387