Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QY069_RS06805 Genome accession   NZ_CP129687
Coordinates   1403360..1403812 (-) Length   150 a.a.
NCBI ID   WP_114064701.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PA2209     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1398360..1408812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QY069_RS06770 - 1398815..1399477 (+) 663 WP_033992423.1 DUF3218 family protein -
  QY069_RS06775 fliP 1399760..1400512 (+) 753 WP_023435066.1 flagellar type III secretion system pore protein FliP -
  QY069_RS06780 fliQ 1400557..1400826 (+) 270 WP_003083053.1 flagellar biosynthesis protein FliQ -
  QY069_RS06785 fliR 1400826..1401603 (+) 778 Protein_1342 flagellar biosynthetic protein FliR -
  QY069_RS06790 - 1401606..1401866 (+) 261 Protein_1343 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  QY069_RS06795 - 1402863..1403105 (+) 243 WP_023082027.1 hypothetical protein -
  QY069_RS06805 pilA 1403360..1403812 (-) 453 WP_114064701.1 pilin Machinery gene
  QY069_RS06810 pilB 1404044..1405744 (+) 1701 WP_010793793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QY069_RS06815 pilC 1405748..1406968 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  QY069_RS06820 pilD 1406972..1407844 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  QY069_RS06825 coaE 1407841..1408452 (+) 612 WP_010793792.1 dephospho-CoA kinase -
  QY069_RS06830 yacG 1408449..1408649 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15471.67 Da        Isoelectric Point: 7.7827

>NTDB_id=854914 QY069_RS06805 WP_114064701.1 1403360..1403812(-) (pilA) [Pseudomonas aeruginosa strain PA2209]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGSQASTEDTTYV
GVDATANKLGTIAVTIGNNGAGTVTFTFAAGNSSPKNSGKIITLTRTDAGSWACTSTQDEMFIPKGCKAP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=854914 QY069_RS06805 WP_114064701.1 1403360..1403812(-) (pilA) [Pseudomonas aeruginosa strain PA2209]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCACTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATCGCTGGGGAAAATATTAAAATTGGTTCCCAAGCATCTACTGAAGATACTACCTATGTA
GGTGTTGATGCTACCGCAAATAAGCTTGGCACTATTGCTGTGACAATTGGGAACAATGGCGCAGGTACCGTGACTTTCAC
TTTTGCAGCCGGCAATTCCAGTCCGAAAAACTCCGGTAAAATTATCACCCTGACCCGGACTGATGCAGGGTCGTGGGCTT
GTACTTCTACCCAGGACGAAATGTTCATCCCTAAAGGTTGCAAAGCGCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.456

99.333

0.68
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.333

100

0.473
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.671

100

0.46
  pilA Vibrio cholerae strain A1552

43.671

100

0.46
  pilA Vibrio cholerae C6706

43.671

100

0.46
  pilA Vibrio parahaemolyticus RIMD 2210633

46.259

98

0.453
  pilA Acinetobacter baumannii strain A118

44.295

99.333

0.44
  pilA2 Legionella pneumophila strain ERS1305867

42.282

99.333

0.42
  comP Acinetobacter baylyi ADP1

40.909

100

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.333

0.413
  pilA2 Legionella pneumophila str. Paris

41.611

99.333

0.413