Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NK8_RS12010 Genome accession   NZ_AP024322
Coordinates   2522036..2523253 (+) Length   405 a.a.
NCBI ID   WP_213226476.1    Uniprot ID   -
Organism   Caballeronia sp. NK8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2517036..2528253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NK8_RS11980 (NK8_23880) cgtA 2517283..2518368 (-) 1086 WP_213226474.1 Obg family GTPase CgtA -
  NK8_RS11985 (NK8_23890) rpmA 2518534..2518794 (-) 261 WP_008342952.1 50S ribosomal protein L27 -
  NK8_RS11990 (NK8_23900) rplU 2518839..2519150 (-) 312 WP_035511298.1 50S ribosomal protein L21 -
  NK8_RS11995 (NK8_23910) - 2519419..2520411 (+) 993 WP_162066353.1 polyprenyl synthetase family protein -
  NK8_RS12005 (NK8_23930) - 2520675..2521961 (+) 1287 WP_213226475.1 HlyC/CorC family transporter -
  NK8_RS12010 (NK8_23940) pilB 2522036..2523253 (+) 1218 WP_213226476.1 GspE/PulE family protein Machinery gene
  NK8_RS12015 (NK8_23950) - 2523250..2524449 (+) 1200 WP_213226477.1 type II secretion system F family protein -
  NK8_RS12020 (NK8_23960) - 2524481..2525401 (+) 921 WP_213226478.1 A24 family peptidase -
  NK8_RS12025 (NK8_23970) coaE 2525419..2526024 (+) 606 WP_213226479.1 dephospho-CoA kinase -
  NK8_RS12030 (NK8_23980) zapD 2526195..2526950 (+) 756 WP_061164498.1 cell division protein ZapD -
  NK8_RS12035 (NK8_23990) - 2527021..2527212 (+) 192 WP_061177722.1 DNA gyrase inhibitor YacG -
  NK8_RS12040 (NK8_24000) - 2527236..2527679 (-) 444 WP_162066359.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43705.06 Da        Isoelectric Point: 7.5327

>NTDB_id=84805 NK8_RS12010 WP_213226476.1 2522036..2523253(+) (pilB) [Caballeronia sp. NK8]
MISPSRDAFDTFARPARSVDPALDSASDHDTPAVSLLADTLREAAERGASDIHVEPSEHDWRIRLRVDGALHVLRRPPPH
LRDAFVTRIKVLARMDIAERRVPQDGRLRLNLPGGRVGDYRVNSLPTLFGEKLVLRRLDALPPDLSLAALGLDDAQSRTV
ESSIRAPHGLVLVTGPTGSGKTLSLYCFLQMLNAEARNVCSVEDPVEIQLAGINQVSVREKAGLTFAVALRAFMRQDPDV
IMVGEIRDAQTADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIGVAPYNLASALRLVTAQRLVRKLCEHCRAPSALPLA
DGFHPFEARGCPACHGIGYRGRVGVHQVMPVSDEIRELIVSRSATHAIARAAQTQGMPALRAAAFARVREGVTSVAEAAE
ATEIE

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=84805 NK8_RS12010 WP_213226476.1 2522036..2523253(+) (pilB) [Caballeronia sp. NK8]
ATGATTTCTCCCTCACGCGACGCTTTCGACACTTTCGCGCGCCCGGCCCGCTCCGTCGATCCCGCGCTCGACAGCGCATC
CGATCACGACACGCCCGCCGTTTCGCTCCTCGCCGACACCCTGCGCGAAGCCGCCGAGCGCGGCGCCTCCGACATTCACG
TCGAACCTTCCGAGCACGACTGGCGCATCCGCCTGCGCGTCGACGGCGCATTGCATGTGCTCAGGCGCCCACCGCCGCAC
TTGCGCGACGCGTTCGTCACGCGGATCAAGGTGCTGGCCCGCATGGATATCGCCGAGCGGCGCGTGCCTCAGGACGGCCG
CCTGCGTCTGAATCTGCCGGGCGGGCGCGTCGGCGACTATCGCGTGAATTCGCTGCCGACGCTCTTCGGCGAAAAGCTCG
TGCTGCGGCGGCTGGACGCGCTGCCGCCCGATCTGTCGCTCGCCGCGCTGGGCCTCGACGACGCGCAAAGCCGCACGGTC
GAATCGTCGATTCGCGCGCCGCACGGTCTCGTGCTCGTGACGGGCCCGACCGGCAGCGGCAAAACCCTGTCGCTCTACTG
CTTCCTGCAGATGCTCAACGCCGAAGCGCGCAACGTGTGCTCCGTCGAAGATCCGGTCGAGATTCAGCTTGCGGGCATCA
ATCAGGTCAGCGTGCGCGAGAAGGCCGGGCTGACGTTCGCCGTGGCGCTGCGCGCGTTCATGCGTCAGGACCCCGACGTC
ATCATGGTCGGCGAGATTCGCGACGCGCAAACCGCGGACGTCGCGGTTAAGGCCGCGCAGACCGGACACCTCGTGCTCTC
GACCTTGCACACGAATGACGCGCCCGCCGCGATCGCGCGGCTCATCGATATCGGCGTCGCGCCTTATAACCTCGCGTCGG
CGCTCAGGCTTGTCACCGCGCAACGGCTCGTGCGCAAGCTGTGCGAGCACTGCCGCGCGCCATCGGCGCTGCCGCTCGCG
GACGGATTTCATCCGTTCGAGGCGCGCGGCTGTCCGGCGTGCCACGGCATCGGCTATCGCGGGCGCGTCGGCGTGCATCA
GGTGATGCCGGTCTCGGACGAGATACGCGAGTTGATCGTCTCGCGGTCGGCGACGCATGCCATCGCGCGCGCCGCACAGA
CGCAAGGCATGCCCGCGCTGCGCGCCGCGGCCTTCGCGCGCGTGCGCGAGGGCGTGACGAGCGTCGCCGAGGCGGCCGAA
GCGACGGAGATCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(40-306)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

49.377

99.012

0.489
  pilF Neisseria gonorrhoeae MS11

47.98

97.778

0.469
  pilB Acinetobacter baumannii D1279779

48.579

95.556

0.464
  pilB Acinetobacter baylyi ADP1

48.312

95.062

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

44.903

100

0.457
  pilB Legionella pneumophila strain ERS1305867

45.226

98.272

0.444
  pilB Vibrio campbellii strain DS40M4

46.615

94.815

0.442
  pilB Vibrio parahaemolyticus RIMD 2210633

45.78

96.543

0.442
  pilF Thermus thermophilus HB27

41.842

93.827

0.393
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.568

95.556

0.388
  pilB Haemophilus influenzae Rd KW20

41.6

92.593

0.385
  pilB Haemophilus influenzae 86-028NP

41.333

92.593

0.383
  pilB Glaesserella parasuis strain SC1401

41.192

91.111

0.375

Multiple sequence alignment