Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QSU95_RS12995 Genome accession   NZ_CP128204
Coordinates   2777564..2779252 (+) Length   562 a.a.
NCBI ID   WP_286211265.1    Uniprot ID   -
Organism   Vibrio furnissii strain VFBJ05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2772564..2784252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSU95_RS12965 (QSU95_12965) dsbC 2772884..2773648 (-) 765 WP_158107763.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  QSU95_RS12970 (QSU95_12970) xerD 2773665..2774573 (-) 909 WP_004729073.1 site-specific tyrosine recombinase XerD -
  QSU95_RS12975 (QSU95_12975) fldB 2774714..2775235 (+) 522 WP_055466520.1 flavodoxin FldB -
  QSU95_RS12980 (QSU95_12980) ampD 2775271..2775825 (-) 555 WP_286211262.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QSU95_RS12985 (QSU95_12985) nadC 2775974..2776861 (+) 888 WP_154180385.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QSU95_RS12990 (QSU95_12990) - 2777108..2777557 (+) 450 WP_055466517.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QSU95_RS12995 (QSU95_12995) pilB 2777564..2779252 (+) 1689 WP_286211265.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QSU95_RS13000 (QSU95_13000) pilC 2779331..2780557 (+) 1227 WP_286211267.1 type II secretion system F family protein Machinery gene
  QSU95_RS13005 (QSU95_13005) pilD 2780609..2781478 (+) 870 WP_014204458.1 A24 family peptidase Machinery gene
  QSU95_RS13010 (QSU95_13010) coaE 2781481..2782089 (+) 609 WP_286211270.1 dephospho-CoA kinase -
  QSU95_RS13015 (QSU95_13015) zapD 2782122..2782862 (+) 741 WP_004729090.1 cell division protein ZapD -
  QSU95_RS13020 (QSU95_13020) yacG 2782920..2783117 (+) 198 WP_004729092.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62045.18 Da        Isoelectric Point: 6.0053

>NTDB_id=846780 QSU95_RS12995 WP_286211265.1 2777564..2779252(+) (pilB) [Vibrio furnissii strain VFBJ05]
MLTNLIAILRQAELISPTQEQAVMDHIHASGATVPEALIDLAIFHHHDLTEQLSQIFGLPETDLTRFDCAPLCQQLGLRE
LITRYQALPLHSNGQILTLAIADPTHLQVEDEFRFATGLQVELVLADHKALHAAIRRLYGRSLATPGTQGKEISQDELAD
LVQISDDEKTTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETLIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVAITPPAALQQQFGLLAED
AVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=846780 QSU95_RS12995 WP_286211265.1 2777564..2779252(+) (pilB) [Vibrio furnissii strain VFBJ05]
ATGCTTACCAACCTTATCGCCATTCTGCGTCAGGCTGAGTTGATCAGCCCAACGCAGGAACAGGCCGTGATGGATCATAT
TCACGCTTCCGGTGCCACGGTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATCTAACCGAGCAACTGA
GTCAGATCTTTGGCCTACCCGAAACCGATCTCACTCGGTTTGATTGCGCGCCGTTGTGCCAGCAGTTGGGACTTCGAGAA
CTCATCACCCGCTACCAAGCCCTGCCGCTGCACAGCAATGGCCAAATCCTGACGTTGGCGATTGCTGATCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCATAAAGCGCTGCACGCCG
CCATTCGCCGCTTGTATGGGCGCAGCCTCGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTAGTACAGATCTCTGACGACGAAAAAACCACCATCGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCGTCCGACATTCACTTTGAGCCGTATGAGTCTCACTACCGTATTCGTC
TGCGCTGTGACGGCATTCTGGTCGAAACCCAACAACCTGCCAGTCACCTCAGTCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAACTGGATATTGCCGAGCGCCGTCTCCCGCAAGATGGACGCATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTGTGGGGTGAAAAGATCGTTCTGCGCCTACTCGACAGTAGCGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAGCAGCAAAAGCAGCTCTACCTCGCGGCCCTCACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACTGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCATCAATCAGGTGCAAGTTCAACCCAAAATCGGCTTTG
GCTTTGCACAAGCACTGCGCTCGTTTCTGCGTCAAGATCCCGATGTGGTGATGGTGGGAGAAATCCGCGATCTAGAAACG
GCAGAAATCGCCATTAAGGCCGCGCAAACTGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCTT
GATTCGTTTAGCCAACATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTCTCTGCCCGCATTGCAAGGTCGCCATCACGCCCCCAGCTGCGCTGCAGCAGCAATTTGGCTTGCTCGCCGAAGAT
GCTGTGTACGAAGCACATCCATCGGGTTGCACGGAATGCACCAATGGGTATAGCGGCCGCGTGGGCATTTATGAAGTGAT
GGCGTTTCATCCCGCCTTGGCGGACGCGGTGATGCAGCGTGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GCATGCAACCCTTGCTGCAATCTGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTACAGCGAGTGCTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.028

100

0.82
  pilB Vibrio campbellii strain DS40M4

71.352

100

0.714
  pilB Vibrio parahaemolyticus RIMD 2210633

71.352

100

0.714
  pilB Legionella pneumophila strain ERS1305867

52.713

91.815

0.484
  pilB Acinetobacter baumannii D1279779

51.252

92.349

0.473
  pilB Acinetobacter baylyi ADP1

50.29

91.993

0.463
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368
  pilF Thermus thermophilus HB27

36.655

100

0.367