Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QSU96_RS13375 Genome accession   NZ_CP128200
Coordinates   2871247..2872935 (+) Length   562 a.a.
NCBI ID   WP_238773636.1    Uniprot ID   -
Organism   Vibrio furnissii strain VFBJ07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2866247..2877935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSU96_RS13345 (QSU96_13345) dsbC 2866563..2867327 (-) 765 WP_055466521.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  QSU96_RS13350 (QSU96_13350) xerD 2867344..2868252 (-) 909 WP_004729073.1 site-specific tyrosine recombinase XerD -
  QSU96_RS13355 (QSU96_13355) fldB 2868394..2868915 (+) 522 WP_004729075.1 flavodoxin FldB -
  QSU96_RS13360 (QSU96_13360) ampD 2868951..2869505 (-) 555 WP_286279616.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QSU96_RS13365 (QSU96_13365) nadC 2869656..2870543 (+) 888 WP_154180385.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QSU96_RS13370 (QSU96_13370) - 2870791..2871240 (+) 450 WP_055466517.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QSU96_RS13375 (QSU96_13375) pilB 2871247..2872935 (+) 1689 WP_238773636.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QSU96_RS13380 (QSU96_13380) pilC 2873014..2874240 (+) 1227 WP_004729085.1 type II secretion system F family protein Machinery gene
  QSU96_RS13385 (QSU96_13385) pilD 2874292..2875161 (+) 870 WP_004729087.1 A24 family peptidase Machinery gene
  QSU96_RS13390 (QSU96_13390) coaE 2875164..2875772 (+) 609 WP_143680340.1 dephospho-CoA kinase -
  QSU96_RS13395 (QSU96_13395) zapD 2875805..2876545 (+) 741 WP_004729090.1 cell division protein ZapD -
  QSU96_RS13400 (QSU96_13400) yacG 2876603..2876800 (+) 198 WP_004729092.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62106.31 Da        Isoelectric Point: 6.1994

>NTDB_id=846732 QSU96_RS13375 WP_238773636.1 2871247..2872935(+) (pilB) [Vibrio furnissii strain VFBJ07]
MLTNLIAILRQAKLISPTQEQAVMDHIHASGATVPEALIDLAIFHHHDLTEQLSQIFGLPETDLTRFDYAPLCQQLGLRE
LITRYQALPLHSNGQILTLAVADPTHLQVEDEFRFATGLQVELVLADHKALHAAIRRLYGRSLATPGTQGKEISQDELAD
LVQISDDEKTTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVAIMPPAALQQQFGLLAED
AVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=846732 QSU96_RS13375 WP_238773636.1 2871247..2872935(+) (pilB) [Vibrio furnissii strain VFBJ07]
ATGCTGACCAACCTTATCGCCATTCTGCGTCAGGCTAAGTTGATCAGCCCAACGCAGGAACAGGCCGTGATGGATCATAT
TCACGCTTCCGGTGCCACGGTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATTTAACCGAGCAACTAA
GTCAGATCTTTGGCCTACCCGAAACCGATCTCACTCGGTTTGATTACGCGCCATTGTGCCAGCAGTTGGGACTTCGAGAA
CTCATCACCCGCTACCAAGCCCTGCCGCTGCACAGCAATGGCCAAATCCTGACGTTGGCGGTTGCTGACCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCATAAAGCGCTGCACGCCG
CTATTCGCCGCTTGTATGGGCGCAGCCTCGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTGGTACAGATCTCTGACGACGAAAAAACCACCATTGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCGTCCGACATTCACTTTGAGCCGTATGAGTCTCACTACCGTATTCGTC
TGCGCTGTGACGGCATTCTGGTCGAAACCCAACAACCTGCCAGTCACCTCAGTCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAACTGGATATTGCCGAGCGCCGCCTCCCGCAAGATGGACGCATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTGTGGGGTGAAAAGATTGTTCTGCGCTTACTCGATAGCAGCGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAACAGCAGAAACAGCTCTACCTCGCAGCCCTCACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACCGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATTTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGTATCAATCAGGTGCAAGTTCAACCCAAAATCGGCTTTG
GCTTTGCACAAGCGCTGCGCTCGTTTCTACGCCAAGATCCCGATGTGGTGATGGTGGGCGAAATTCGCGATCTAGAAACC
GCAGAAATCGCCATTAAAGCCGCGCAAACCGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCGT
GATTCGTTTAGCCAACATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTCTCTGCCCGCATTGCAAGGTCGCCATTATGCCCCCAGCGGCGCTGCAGCAGCAATTTGGCTTGCTCGCCGAAGAT
GCCGTGTACGAAGCACATCCATCGGGTTGCACGGAATGCACCAATGGGTATAGCGGCCGCGTGGGCATTTATGAAGTGAT
GGCGTTTCATCCCGCCTTGGCGGACGCGGTGATGCAGCGCGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GCATGCAACCCTTGCTGCAATCTGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTGCAGCGAGTACTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.384

100

0.824
  pilB Vibrio campbellii strain DS40M4

71.886

100

0.719
  pilB Vibrio parahaemolyticus RIMD 2210633

71.708

100

0.717
  pilB Legionella pneumophila strain ERS1305867

52.805

91.993

0.486
  pilB Acinetobacter baumannii D1279779

51.06

92.349

0.472
  pilB Acinetobacter baylyi ADP1

50.097

91.993

0.461
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368
  pilF Thermus thermophilus HB27

36.655

100

0.367