Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QQS40_RS00240 Genome accession   NZ_CP127167
Coordinates   48472..49851 (+) Length   459 a.a.
NCBI ID   WP_329505451.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain HP01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 43472..54851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQS40_RS00205 - 43766..44617 (-) 852 WP_329505438.1 thymidylate synthase -
  QQS40_RS00210 lgt 44628..45437 (-) 810 WP_329505440.1 prolipoprotein diacylglyceryl transferase -
  QQS40_RS00215 - 45446..46240 (-) 795 WP_329505442.1 sulfite exporter TauE/SafE family protein -
  QQS40_RS00220 rppH 46240..46833 (-) 594 WP_329505444.1 RNA pyrophosphohydrolase -
  QQS40_RS00225 ampD 47318..47881 (-) 564 WP_329505446.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QQS40_RS00230 - 47874..48032 (-) 159 WP_329505448.1 hypothetical protein -
  QQS40_RS00235 pilA 48022..48465 (+) 444 WP_075875764.1 prepilin peptidase-dependent pilin Machinery gene
  QQS40_RS00240 pilB 48472..49851 (+) 1380 WP_329505451.1 GspE/PulE family protein Machinery gene
  QQS40_RS00245 pilC 49860..51083 (+) 1224 WP_128786982.1 type II secretion system F family protein Machinery gene
  QQS40_RS00250 pilD 51080..51757 (+) 678 WP_329505454.1 A24 family peptidase Machinery gene
  QQS40_RS00255 coaE 51843..52463 (+) 621 WP_329505456.1 dephospho-CoA kinase -
  QQS40_RS00260 yacG 52456..52662 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  QQS40_RS00265 - 52659..52940 (+) 282 WP_329505459.1 GNAT family N-acetyltransferase -
  QQS40_RS00270 rhlB 53031..54290 (+) 1260 WP_289901439.1 ATP-dependent RNA helicase RhlB -
  QQS40_RS00275 mreD 54331..54819 (-) 489 WP_149785850.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52123.29 Da        Isoelectric Point: 5.9072

>NTDB_id=842301 QQS40_RS00240 WP_329505451.1 48472..49851(+) (pilB) [Haemophilus parainfluenzae strain HP01]
MAYQATAKNGEIYQISPQYWQQNQQQQALLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETHQLK
QLLQSLAPKANQIEEQATFYHHSEEESTANLSNDDEPVIQLLNGIFETALQKNASDIHLETQPNGLLVRLRIDGVLQPQP
IISKSLANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQN
QQSQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQSHEIKNSLLLVIAQRLVRKRYLKCSQEF
SDSCDCHQGYQGRIGVYQFLQCENNRYQTDFDSLYQSALEKVKDNITDLDEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=842301 QQS40_RS00240 WP_329505451.1 48472..49851(+) (pilB) [Haemophilus parainfluenzae strain HP01]
ATGGCTTATCAAGCAACGGCAAAAAATGGCGAGATTTATCAAATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCTTTGCTGTTACGTTACTTTGCGCTCCCTCTTAAAGAAGACGAACACCATTTATGGCTTGCGGTGGATTCGCTGAATA
ATCTTGCAGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTAGTTGAACCGATTTTATTTGAAACACATCAACTCAAA
CAACTTTTACAATCCCTTGCACCGAAAGCCAATCAAATAGAAGAACAAGCGACGTTTTATCATCATTCAGAAGAGGAAAG
CACCGCTAATTTATCTAATGATGATGAACCCGTTATTCAATTATTAAATGGTATTTTTGAAACCGCCCTGCAAAAAAATG
CCTCCGATATTCATTTAGAAACCCAGCCAAATGGGCTTTTAGTTCGTTTGCGTATTGATGGTGTACTTCAACCACAGCCG
ATTATCAGTAAATCGCTCGCCAATCGTGTGATTTCTCGTTTAAAACTCTTAGCCAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGCTTTCAATTCAAAACGACGTTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACGCATT
GGGGAGAAAAAGCGGTATTACGATTACAACAAAATAAACCGGTTCAATTAAGTTTTGCTGAACTTGGCATGACACAGAAC
CAACAAAGTCAATTTCAACATGCACTCAGCCAACCGCAAGGTTTAATCCTCGTGACTGGACCAACGGGAAGCGGAAAAAG
TATCTCACTTTATACCGCACTCCAATGGCTCAATACGCCAGATAAACACATTATGACGGCAGAAGATCCCGTTGAAATAG
AATTAGAAGGCATCATTCAAACTCAAGTGAATCCGCAAATCGGTTTAGATTTTAGTCGATTACTTCGTACATTTTTACGG
CAAGATCCCGACATTATTATGCTTGGTGAAATTCGAGACGAAGAAAGTGCATTAATGGCATTAAGAGCCGCTCAAACCGG
CCATCTAGTACTTTCTACCTTGCATACCAATGATGCCATCTCAGCCATTTCGCGCTTACAGCAATTAGGCATTCAATCTC
ACGAAATTAAAAATAGTCTCTTATTAGTTATCGCACAGCGATTGGTGCGCAAACGTTACTTAAAGTGCAGTCAAGAGTTT
AGTGATTCTTGTGATTGCCATCAAGGGTATCAAGGGCGTATTGGGGTGTATCAATTTTTACAATGCGAAAACAACCGTTA
CCAAACTGATTTCGATTCACTTTATCAAAGCGCGTTAGAAAAAGTAAAAGATAACATCACCGATCTTGATGAGATTCAAC
GCGTGTTAGGCAAAAAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-88)

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

77.802

99.129

0.771
  pilB Haemophilus influenzae Rd KW20

77.582

99.129

0.769
  pilB Glaesserella parasuis strain SC1401

59.292

98.475

0.584
  pilB Vibrio campbellii strain DS40M4

41.116

100

0.434
  pilB Vibrio cholerae strain A1552

41.25

100

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

41.25

100

0.431
  pilB Acinetobacter baylyi ADP1

38.554

100

0.418
  pilB Acinetobacter baumannii D1279779

39.014

100

0.414
  pilB Legionella pneumophila strain ERS1305867

38.446

100

0.41
  pilF Neisseria gonorrhoeae MS11

36.82

100

0.383
  pilF Thermus thermophilus HB27

38.928

93.464

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.095

100

0.362