Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QPX86_RS06915 Genome accession   NZ_CP126972
Coordinates   1645311..1646423 (+) Length   370 a.a.
NCBI ID   WP_220753528.1    Uniprot ID   -
Organism   Shewanella goraebulensis strain DAU312     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1640311..1651423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPX86_RS06885 - 1640684..1641130 (-) 447 WP_285164714.1 DUF4426 domain-containing protein -
  QPX86_RS06890 - 1641314..1641556 (-) 243 WP_285165146.1 DUF167 family protein -
  QPX86_RS06895 - 1641892..1642440 (-) 549 WP_285164715.1 YggT family protein -
  QPX86_RS06900 proC 1642514..1643332 (-) 819 WP_285164716.1 pyrroline-5-carboxylate reductase -
  QPX86_RS06905 - 1643537..1644226 (-) 690 WP_285164717.1 YggS family pyridoxal phosphate-dependent enzyme -
  QPX86_RS06910 pilT 1644262..1645299 (+) 1038 WP_220753529.1 type IV pilus twitching motility protein PilT Machinery gene
  QPX86_RS06915 pilU 1645311..1646423 (+) 1113 WP_220753528.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QPX86_RS06920 - 1646885..1647430 (+) 546 WP_285164718.1 glutathione peroxidase -
  QPX86_RS06925 hemH 1647591..1648604 (+) 1014 WP_285164719.1 ferrochelatase -
  QPX86_RS06930 ruvX 1648725..1649150 (-) 426 WP_285164720.1 Holliday junction resolvase RuvX -
  QPX86_RS06935 - 1649343..1649903 (-) 561 WP_220753524.1 YqgE/AlgH family protein -
  QPX86_RS06940 yciH 1649982..1650311 (-) 330 WP_285164721.1 stress response translation initiation inhibitor YciH -
  QPX86_RS06945 - 1650592..1651152 (+) 561 WP_285164722.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41187.19 Da        Isoelectric Point: 6.2362

>NTDB_id=840893 QPX86_RS06915 WP_220753528.1 1645311..1646423(+) (pilU) [Shewanella goraebulensis strain DAU312]
MDVRPFLKVMVDRKASDLFVTAGFPPSAKIDGELRPLSESSFNPEQSLDFVESLMTDVQKQEFHDTRECNFAFAAKELGR
FRVSAFWQRESPGCVMRRIETKIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLASLVGYRNAHARGHILTIED
PVEFVHNHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHNQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHLLKETMSKSREQGMQTFDQA
LLDLYLEKEITYADALHHADSPNDLRLMIKLQNNETSGSGLMDGVTLDLG

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=840893 QPX86_RS06915 WP_220753528.1 1645311..1646423(+) (pilU) [Shewanella goraebulensis strain DAU312]
ATGGATGTACGTCCTTTTTTAAAGGTCATGGTTGACCGTAAAGCCTCTGATTTATTTGTTACAGCGGGCTTTCCGCCGAG
TGCAAAAATTGATGGTGAATTAAGACCGCTTTCTGAGTCTAGCTTTAACCCTGAACAGTCGTTAGATTTTGTTGAATCGC
TAATGACTGATGTTCAAAAACAAGAGTTTCATGACACTCGAGAATGTAATTTTGCATTTGCAGCAAAAGAGTTAGGCCGT
TTTCGTGTCAGTGCCTTTTGGCAGCGTGAATCTCCTGGTTGCGTAATGCGTCGTATTGAAACCAAAATTCCTGAAGTAGA
AGATTTAAAACTACCTCCAATTTTGAAAGATTTGGTGATGAGTAAGCGCGGTCTTATTATCATGGTCGGTGGTACGGGTA
CGGGTAAATCTACATCATTGGCATCTTTAGTCGGTTATCGTAATGCGCACGCGCGTGGGCATATTTTAACCATTGAAGAC
CCTGTGGAATTTGTTCATAACCACCGTAAAAGCATTATTACTCAGCGCGAAGTTGGTATTGATACCGACTCATTTGATGC
GGCACTTAAAAGCTCGCTGCGTCAAGCGCCAGATGTTATTTTGATTGGTGAGATTCGAACTCAAGAGACCATGGAGTTTG
CGTTATCTTTTGCTGAAACTGGTCATTTATGTATGGCAACACTTCATGCTAACAACGCTAACCAAGCATTAGATCGTATT
ATGCATTTAGTACCTGAAAGTAAACATAACCAGTTATTATTTGATTTGTCACTTAACTTACGCGGTATTGTCGCGCAGCA
ATTAGTGCCTAAATCCGATGGTTCAGGTCGTCGTGCGGCGATTGAAGTACTCATTAATACCCCTCGTGTAGCCAGCTTAA
TAGCGAAAAATGAATTGCACTTACTCAAAGAAACGATGAGTAAATCACGCGAACAAGGCATGCAGACCTTTGATCAAGCA
TTATTAGATTTATATTTGGAAAAAGAAATTACCTATGCAGATGCATTGCATCATGCTGATTCTCCGAATGACTTACGTCT
AATGATTAAGCTTCAAAATAATGAAACCAGTGGTTCAGGTTTAATGGATGGCGTGACATTAGATTTAGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.484

100

0.608
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilU Vibrio cholerae strain A1552

56.64

99.73

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.947

86.216

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.947

86.216

0.37
  pilT Legionella pneumophila strain Lp02

40.118

91.622

0.368
  pilT Legionella pneumophila strain ERS1305867

40.118

91.622

0.368