Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB   Type   Machinery gene
Locus tag   JG741_RS06395 Genome accession   NZ_AP024210
Coordinates   1248000..1248389 (-) Length   129 a.a.
NCBI ID   WP_021165898.1    Uniprot ID   A0A166Z4Y5
Organism   Lactococcus cremoris strain G3-2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1240069..1248389 1248000..1248389 within 0


Gene organization within MGE regions


Location: 1240069..1248389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG741_RS06355 (LLG32_13290) ricT 1240069..1240851 (-) 783 WP_011834464.1 stage 0 sporulation family protein -
  JG741_RS06360 (LLG32_13300) - 1240901..1241761 (-) 861 WP_061777746.1 DNA polymerase III subunit delta' -
  JG741_RS06365 (LLG32_13310) tmk 1241894..1242529 (-) 636 WP_015082045.1 dTMP kinase -
  JG741_RS06370 (LLG32_13320) yycF 1242707..1243408 (+) 702 WP_003131580.1 response regulator YycF -
  JG741_RS06375 (LLG32_13330) - 1243401..1244828 (+) 1428 WP_061777747.1 sensor histidine kinase -
  JG741_RS06380 (LLG32_13350) - 1245018..1245830 (+) 813 WP_061777748.1 MBL fold metallo-hydrolase -
  JG741_RS06385 (LLG32_13360) groL 1245882..1247510 (-) 1629 WP_032950921.1 chaperonin GroEL -
  JG741_RS06390 (LLG32_13370) groES 1247597..1247881 (-) 285 WP_011675464.1 co-chaperone GroES -
  JG741_RS06395 (LLG32_13380) ssbB 1248000..1248389 (-) 390 WP_021165898.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 129 a.a.        Molecular weight: 14703.77 Da        Isoelectric Point: 6.9865

>NTDB_id=84082 JG741_RS06395 WP_021165898.1 1248000..1248389(-) (ssbB) [Lactococcus cremoris strain G3-2]
MNKTMLIGRLTNAPEISKTTNNKSYVRVTLAVNRRFKNEKGEREADFISIILWGKSAETFVSYAKKGSLISVEGEIRTRN
YTDKNEQKHCITEILGLSYDLLESRATLALRESAVNFEELLLEADELPF

Nucleotide


Download         Length: 390 bp        

>NTDB_id=84082 JG741_RS06395 WP_021165898.1 1248000..1248389(-) (ssbB) [Lactococcus cremoris strain G3-2]
ATGAATAAAACCATGTTGATTGGTCGTTTAACAAACGCACCTGAAATTTCGAAAACAACGAACAATAAATCTTATGTCCG
TGTGACTCTGGCAGTCAATCGCCGCTTCAAAAATGAAAAAGGAGAGCGAGAAGCAGACTTTATCTCGATAATTTTATGGG
GAAAATCAGCAGAAACATTCGTTTCCTATGCTAAAAAAGGAAGTCTTATTTCTGTAGAGGGAGAGATTAGAACTAGAAAT
TATACTGATAAAAATGAACAGAAACACTGTATTACAGAAATCTTAGGTTTGAGCTATGATTTATTGGAAAGTCGTGCGAC
TCTTGCCTTGAGAGAAAGCGCTGTAAATTTTGAGGAACTCTTACTTGAAGCTGATGAGCTCCCTTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A166Z4Y5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Lactococcus lactis subsp. cremoris KW2

96.899

100

0.969

  ssbB Streptococcus sobrinus strain NIDR 6715-7

60

100

0.605

  ssbA Streptococcus mutans UA159

56.154

100

0.566

  ssbB/cilA Streptococcus pneumoniae TIGR4

56.154

100

0.566

  ssbB/cilA Streptococcus mitis SK321

55.385

100

0.558

  ssbB/cilA Streptococcus pneumoniae R6

55.385

100

0.558

  ssbB/cilA Streptococcus pneumoniae Rx1

55.385

100

0.558

  ssbB/cilA Streptococcus pneumoniae D39

55.385

100

0.558

  ssbB/cilA Streptococcus mitis NCTC 12261

55.385

100

0.558

  ssb Latilactobacillus sakei subsp. sakei 23K

41.964

86.822

0.364


Multiple sequence alignment