Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QMY49_RS01150 Genome accession   NZ_CP125875
Coordinates   225217..225654 (-) Length   145 a.a.
NCBI ID   WP_029790345.1    Uniprot ID   -
Organism   Vibrio harveyi strain SB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 220217..230654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMY49_RS01130 (QMY49_01130) coaE 220706..221320 (-) 615 WP_017817675.1 dephospho-CoA kinase -
  QMY49_RS01135 (QMY49_01135) pilD 221321..222190 (-) 870 WP_005446255.1 A24 family peptidase Machinery gene
  QMY49_RS01140 (QMY49_01140) pilC 222263..223486 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  QMY49_RS01145 (QMY49_01145) pilB 223520..225205 (-) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMY49_RS01150 (QMY49_01150) pilA 225217..225654 (-) 438 WP_029790345.1 pilin Machinery gene
  QMY49_RS01155 (QMY49_01155) nadC 225920..226807 (-) 888 WP_283312494.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QMY49_RS01160 (QMY49_01160) ampD 226900..227451 (+) 552 WP_017189135.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QMY49_RS01165 (QMY49_01165) pdhR 227856..228623 (+) 768 WP_009699648.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14897.02 Da        Isoelectric Point: 9.7639

>NTDB_id=833229 QMY49_RS01150 WP_029790345.1 225217..225654(-) (pilA) [Vibrio harveyi strain SB1]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAVPQYQKYVAKSEAASALASITGHRINVETYVVENGSFPTAAQLPVPTSP
LGVISYTASASGASSSSGAIKFTFNSSGVSPDVISKDVTLGRDGTGQWNCTSGITATGVKPKACS

Nucleotide


Download         Length: 438 bp        

>NTDB_id=833229 QMY49_RS01150 WP_029790345.1 225217..225654(-) (pilA) [Vibrio harveyi strain SB1]
ATGAAAACGAATAAACAGAAGAAGCAGCAGGGTTTTACTCTAATTGAATTGATGATCGTGGTAGCGGTTATTGGTGTTTT
GGCAGCGATTGCCGTCCCGCAGTACCAAAAATACGTAGCTAAAAGTGAAGCAGCTTCAGCACTAGCATCAATTACTGGAC
ATAGAATAAATGTTGAAACTTATGTTGTTGAAAATGGATCATTTCCGACAGCAGCACAGCTACCTGTTCCGACCTCACCA
CTCGGTGTAATTAGTTACACTGCCTCAGCTTCAGGAGCAAGCTCATCATCAGGTGCAATAAAGTTTACGTTTAATAGTTC
AGGTGTAAGTCCAGACGTAATCAGCAAAGACGTAACCCTTGGGCGAGATGGTACTGGTCAATGGAACTGCACCTCTGGCA
TCACTGCTACAGGTGTAAAACCTAAAGCATGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.403

100

0.503
  pilA Vibrio cholerae strain A1552

47.403

100

0.503
  pilA Vibrio cholerae C6706

47.403

100

0.503
  pilA Vibrio parahaemolyticus RIMD 2210633

42.667

100

0.441
  pilA Pseudomonas aeruginosa PAK

42.282

100

0.434
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.096

100

0.414
  pilA Acinetobacter baumannii strain A118

41.606

94.483

0.393
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.571

96.552

0.372