Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QLH32_RS10850 Genome accession   NZ_CP125669
Coordinates   2281347..2282513 (-) Length   388 a.a.
NCBI ID   WP_283266236.1    Uniprot ID   -
Organism   Acinetobacter sp. KCTC 92772     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2276347..2287513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLH32_RS10820 (QLH32_10820) rpmH 2276552..2276686 (+) 135 WP_000831329.1 50S ribosomal protein L34 -
  QLH32_RS10825 (QLH32_10825) rnpA 2276714..2277109 (+) 396 WP_283266231.1 ribonuclease P protein component -
  QLH32_RS10830 (QLH32_10830) yidD 2277119..2277439 (+) 321 WP_283266232.1 membrane protein insertion efficiency factor YidD -
  QLH32_RS10835 (QLH32_10835) yidC 2277445..2279202 (+) 1758 WP_283266233.1 membrane protein insertase YidC -
  QLH32_RS10840 (QLH32_10840) mnmE 2279317..2280672 (+) 1356 WP_283266234.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  QLH32_RS10845 (QLH32_10845) - 2280864..2281211 (+) 348 WP_283266235.1 hypothetical protein -
  QLH32_RS10850 (QLH32_10850) pilU 2281347..2282513 (-) 1167 WP_283266236.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QLH32_RS10855 (QLH32_10855) - 2283164..2284312 (+) 1149 WP_283266237.1 HlyD family secretion protein -
  QLH32_RS10860 (QLH32_10860) - 2284337..2285875 (+) 1539 WP_283266238.1 DHA2 family efflux MFS transporter permease subunit -
  QLH32_RS10865 (QLH32_10865) - 2285970..2286776 (+) 807 WP_279960820.1 hypothetical protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 44136.65 Da        Isoelectric Point: 6.7415

>NTDB_id=832348 QLH32_RS10850 WP_283266236.1 2281347..2282513(-) (pilU) [Acinetobacter sp. KCTC 92772]
MYNAELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTRLFAYSLMNEKQRQEFENEWEC
NFAINVPNVSRFRVNVFKQQLQTGMVIRTITSEIPTFQKLKLPESLKNVMLEKRGLVLVVGSTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMVTHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGKGRRAAVEILLNTPLIAENILKGHFHNLKEIMTKS
RELGMQTFDQALFDLYNEGDISYDEALRNADSVNELRLQIKLKGNRPETSVVMTPLNMVKEETEQPEP

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=832348 QLH32_RS10850 WP_283266236.1 2281347..2282513(-) (pilU) [Acinetobacter sp. KCTC 92772]
ATGTACAACGCGGAACTACTAGAAGAAGCCAAAACCTTTATGTTTCACATGCTCACCAAGGTGGTGGAGTATGGAGGATC
GGATTTATTCATTACGGCGGATTTTCCCCCAAGCATTAAATTGCAGGGCCTGATGCGCCCCTTGGGACAGCAGGCATTAA
CAGCGGAAAAAACCAGGTTGTTTGCCTATAGTCTGATGAATGAAAAGCAGCGGCAGGAATTTGAAAATGAATGGGAATGT
AATTTTGCCATCAATGTTCCCAACGTTTCACGCTTCCGTGTCAATGTGTTTAAGCAGCAACTCCAGACAGGGATGGTGAT
TCGAACCATCACTTCCGAAATTCCAACGTTTCAAAAGCTGAAACTTCCAGAATCCCTTAAAAATGTCATGCTGGAAAAAC
GTGGTCTGGTCTTGGTGGTGGGAAGTACAGGCTCAGGAAAATCAACTTCCTTGGCTGCAATGATTGACCATCGTAACGAA
AACTCGGCAGGGCATATCATTACCGTTGAAGACCCAGTGGAATATGTGCACAAGCATAAAAAATCGATGGTGACCCACCG
TGAAGTTGGGGTTGACTGCCACACCTGGCACCATGCCTTGAAAAATACCCTCCGTCAGGCGCCTGACGTGATCCTGATTG
GTGAAATCCGTGATACCGAAACCATGGAGCATGCGATTGCCTTTGCCGAAACAGGGCATCTCTGCCTAGGCACGCTACAT
GCCAACAATGCCAACCAGGCCCTTGACCGTATTATCAACTTCTTCCCTGAAGAACGCCGCAACCAACTGCTGATGGATTT
ATCATCCAACATGAAAGCTATTATTTCCCAGCGTTTGGTACGCACCCAGGATGGTAAAGGCCGCCGTGCAGCGGTAGAAA
TTCTACTCAACACCCCCTTGATTGCCGAAAATATACTCAAAGGCCACTTCCATAACTTGAAAGAGATTATGACCAAATCC
CGCGAACTGGGTATGCAAACATTCGATCAGGCGCTGTTTGATTTATACAATGAGGGAGATATCAGCTACGATGAGGCATT
GCGCAATGCCGATTCCGTGAATGAATTGCGTTTACAGATTAAGTTAAAAGGCAATCGCCCAGAAACCTCAGTTGTCATGA
CACCCCTGAATATGGTGAAAGAAGAAACTGAACAACCAGAGCCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(91-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.272

89.175

0.546
  pilU Acinetobacter baylyi ADP1

54.802

91.237

0.5
  pilU Vibrio cholerae strain A1552

52.89

89.175

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.515

86.082

0.366
  pilT Acinetobacter nosocomialis M2

43.252

84.021

0.363
  pilT Acinetobacter baumannii D1279779

43.252

84.021

0.363
  pilT Acinetobacter baumannii strain A118

43.252

84.021

0.363
  pilT Acinetobacter baylyi ADP1

41.667

86.598

0.361
  pilT Pseudomonas aeruginosa PAK

41.916

86.082

0.361