Detailed information NTG0831544

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QMK51_RS11800
• Genome accession: NZ_CP125375
• Coordinates: 2640873..2641982 (+)
• Length: 369 a.a.
• NCBI ID: WP_320394139.1
• Uniprot ID: -
• Organism: Pseudomonas sp. P9_31
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2635873..2646982

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QMK51_RS11785 (QMK51_11780)	-	2636799..2637731 (+)	933	WP_320394137.1	sugar kinase	-
QMK51_RS11790 (QMK51_11785)	-	2637875..2638150 (-)	276	WP_007933743.1	peptidylprolyl isomerase	-
QMK51_RS11795 (QMK51_11790)	-	2638216..2640753 (-)	2538	WP_320394138.1	PAS domain S-box protein	-
QMK51_RS11800 (QMK51_11795)	pilU	2640873..2641982 (+)	1110	WP_320394139.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QMK51_RS11805 (QMK51_11800)	-	2642106..2644061 (-)	1956	WP_320394140.1	acetoacetate--CoA ligase	-
QMK51_RS11810 (QMK51_11805)	-	2644197..2644982 (-)	786	WP_320394141.1	3-hydroxybutyrate dehydrogenase	-
QMK51_RS11815 (QMK51_11810)	-	2644990..2646381 (-)	1392	WP_320394142.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40724.59 Da
• Isoelectric Point: 6.6448

>NTDB_id=831544 QMK51_RS11800 WP_320394139.1 2640873..2641982(+) (pilU) [Pseudomonas sp. P9_31]
MEIDALLQILASRNGSDLYLSTGAPPSAKFDGVLKPLTDQPFKPGEVAAIAASIMDAEQRLEFDRDLEMNLALSLPGVGR
FRLSIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLETVMLKQGLILFVGSTGSGKSTSLAALIDYRNRHSSGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRGQLLNDLGNNLKAFVSQRLVRTRDGQRRAAVEVMVGTPTIGDLIRRNEFGELKGIMEKSSEAGMQTFDGAL
FALFAEGAIDEAEALKHADSVNNLRLRLKMHAEATPGPHTPPGEWGLVD

Nucleotide

• Length: 1110 bp

>NTDB_id=831544 QMK51_RS11800 WP_320394139.1 2640873..2641982(+) (pilU) [Pseudomonas sp. P9_31]
ATGGAAATCGATGCACTGTTGCAAATTCTGGCCAGCCGCAATGGCTCCGATCTTTACCTGTCCACGGGCGCGCCACCCAG
CGCGAAATTTGACGGTGTGCTCAAACCCCTGACTGATCAACCGTTCAAGCCGGGAGAAGTGGCGGCTATTGCCGCGTCCA
TCATGGACGCCGAACAGCGCCTGGAGTTCGATCGGGACCTGGAAATGAACCTGGCGCTTTCCCTGCCCGGGGTCGGGCGT
TTCCGGCTCAGTATCTTCAAGCAGCGCAATGATGTGTCGATTGTGGCGCGCAACATCAAACTCGACATTCCACGTTTTGA
AGACCTCAAGCTGCCGCCAGTGCTGCTCGAAACCGTGATGCTCAAACAGGGGCTGATACTGTTCGTCGGTTCGACCGGTT
CGGGTAAGTCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCCACAGCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAGTTCATCCATCGGCACAAGAAGTCTATCGTCAATCAGCGTGAGGTTGGCGTCGATACCCGCAGTTTTCATGC
TGCGTTGAAAAATACCCTGCGCCAGGCACCGGACGTGGTGCTGATCGGCGAGATCCGAGACCGCGAAACCATGGAGCACG
CGCTGGCGTTTGCCGATACCGGTCATCTGGTGATTTCCACCTTGCATGCGCATAACGCCAATCAGGCGTTGGATCGGGTG
ATCAATTTCTTTCCCGAAGAGCGCCGTGGGCAGTTGCTCAACGACCTGGGTAACAACCTCAAGGCGTTTGTTTCCCAGCG
TTTGGTGCGCACCCGCGATGGGCAACGCCGGGCAGCGGTGGAAGTGATGGTGGGCACGCCGACTATCGGCGACCTGATCC
GGCGCAACGAGTTTGGCGAGCTCAAGGGGATCATGGAGAAGTCTTCGGAGGCGGGGATGCAGACGTTTGACGGGGCACTG
TTCGCGCTGTTTGCCGAGGGCGCGATCGATGAGGCAGAGGCGCTGAAACATGCGGACTCGGTGAACAACTTGCGCCTGCG
CCTGAAAATGCACGCCGAGGCCACGCCCGGGCCCCATACACCGCCGGGTGAATGGGGTTTGGTGGACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.285	100	0.553
pilU	Acinetobacter baylyi ADP1	53.371	96.477	0.515
pilU	Vibrio cholerae strain A1552	50.571	94.851	0.48
pilT	Pseudomonas stutzeri DSM 10701	40.698	93.225	0.379
pilT	Pseudomonas aeruginosa PAK	40.407	93.225	0.377
pilT	Vibrio cholerae strain A1552	41.141	90.244	0.371
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	90.244	0.371
pilT	Legionella pneumophila strain Lp02	40.12	90.515	0.363
pilT	Legionella pneumophila strain ERS1305867	40.12	90.515	0.363