Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   OPT59_RS00730 Genome accession   NZ_CP125360
Coordinates   119731..120672 (+) Length   313 a.a.
NCBI ID   WP_015016543.1    Uniprot ID   A0AB38Y1A8
Organism   Streptococcus dysgalactiae subsp. equisimilis strain UT_120444     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114731..125672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OPT59_RS00720 (OPT59_00720) rpoC 115441..119082 (+) 3642 WP_015016542.1 DNA-directed RNA polymerase subunit beta' -
  OPT59_RS00725 (OPT59_00725) - 119285..119650 (+) 366 WP_003055257.1 DUF1033 family protein -
  OPT59_RS00730 (OPT59_00730) comGA/cglA 119731..120672 (+) 942 WP_015016543.1 competence type IV pilus ATPase ComGA Machinery gene
  OPT59_RS00735 (OPT59_00735) comYB 120608..121639 (+) 1032 WP_042357807.1 competence type IV pilus assembly protein ComGB Machinery gene
  OPT59_RS00740 (OPT59_00740) comYC 121641..121967 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  OPT59_RS00745 (OPT59_00745) comGD 121942..122370 (+) 429 WP_041788719.1 competence type IV pilus minor pilin ComGD -
  OPT59_RS00750 (OPT59_00750) comGE 122378..122623 (+) 246 WP_003058569.1 competence type IV pilus minor pilin ComGE -
  OPT59_RS00755 (OPT59_00755) comGF 122604..123044 (+) 441 WP_003061780.1 competence type IV pilus minor pilin ComGF -
  OPT59_RS00760 (OPT59_00760) comGG 123022..123396 (+) 375 WP_012766449.1 competence type IV pilus minor pilin ComGG -
  OPT59_RS00765 (OPT59_00765) comYH 123459..124412 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  OPT59_RS00770 (OPT59_00770) - 124471..125667 (+) 1197 WP_003058485.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35194.33 Da        Isoelectric Point: 7.2836

>NTDB_id=831250 OPT59_RS00730 WP_015016543.1 119731..120672(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain UT_120444]
MVQALARSILKEAEQIQAQDIYILPKEDQYELLIRVGDERRLVDVYRGDRMVHLISHFKFVAGMTVGEKRRCQVGSCDYD
IGGDTLLSLRLSSVGNYRGQESLVIRLLHHQQRSLHYWFDGLKTVARQIGGRGLYLFAGPVGSGKTTLMHQLIADYYQGA
QVISIEDPVEIKNHQALQLQVNDSIGMTYDNLIKLSLRHRPDILIIGEIRDSQTARAVIRASLTGVLVFSTVHAKSISGV
YARLLELGVTRAELDNCLALVAYQRLLNGGALIDSTQTEFEHYSPNQWNQKIDDLLAAGHLSPEQARFEKIIQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=831250 OPT59_RS00730 WP_015016543.1 119731..120672(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain UT_120444]
ATGGTACAAGCATTAGCAAGATCTATTTTGAAAGAAGCTGAGCAGATTCAAGCTCAAGATATCTATATTTTGCCAAAGGA
AGATCAGTATGAGCTATTGATAAGGGTAGGAGATGAAAGGAGATTGGTGGATGTTTATCGGGGCGATCGGATGGTTCATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGACCGTTGGTGAGAAACGACGCTGTCAAGTCGGTTCTTGTGATTATGAT
ATCGGTGGAGACACACTTTTGTCTCTACGCCTGTCTAGTGTTGGAAATTACCGTGGACAAGAGAGCCTAGTGATTCGGTT
ATTACATCATCAACAAAGAAGTTTACATTACTGGTTTGACGGACTGAAAACAGTTGCACGTCAGATTGGAGGCCGAGGAC
TCTATCTTTTTGCAGGTCCAGTCGGATCAGGCAAAACGACTTTGATGCACCAATTGATTGCTGATTATTATCAAGGAGCA
CAGGTCATTAGCATAGAAGATCCGGTGGAAATTAAAAATCATCAAGCGCTCCAATTACAAGTTAATGATAGTATTGGCAT
GACTTACGACAACTTGATCAAACTATCTTTACGGCATCGCCCTGATATTTTAATCATTGGTGAAATTCGAGACAGCCAGA
CAGCTAGAGCGGTTATTAGAGCAAGTTTAACAGGTGTCTTAGTATTTTCAACAGTTCATGCCAAAAGCATTTCTGGTGTT
TATGCCAGATTACTAGAACTAGGAGTGACAAGAGCAGAATTAGACAATTGTTTGGCTTTGGTTGCTTACCAACGATTGCT
TAATGGAGGAGCATTGATTGACTCAACTCAAACAGAATTTGAACACTACTCACCAAACCAATGGAATCAGAAAATTGATG
ACCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.613

99.042

0.61
  comYA Streptococcus mutans UA140

61.093

99.361

0.607
  comYA Streptococcus mutans UA159

61.093

99.361

0.607
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.361

0.604
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.486

99.361

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

52.09

99.361

0.518
  comGA Latilactobacillus sakei subsp. sakei 23K

38.908

93.61

0.364