Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMA14_RS00265 Genome accession   NZ_CP125087
Coordinates   56722..58101 (+) Length   459 a.a.
NCBI ID   WP_284173946.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain EL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 51722..63101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMA14_RS00235 (QMA14_00235) - 52014..52865 (-) 852 WP_115912286.1 thymidylate synthase -
  QMA14_RS00240 (QMA14_00240) lgt 52876..53685 (-) 810 WP_115912284.1 prolipoprotein diacylglyceryl transferase -
  QMA14_RS00245 (QMA14_00245) - 53694..54488 (-) 795 WP_005695514.1 sulfite exporter TauE/SafE family protein -
  QMA14_RS00250 (QMA14_00250) rppH 54488..55081 (-) 594 WP_049376123.1 RNA pyrophosphohydrolase -
  QMA14_RS00255 (QMA14_00255) ampD 55566..56129 (-) 564 WP_005695512.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QMA14_RS00260 (QMA14_00260) pilA 56272..56715 (+) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  QMA14_RS00265 (QMA14_00265) pilB 56722..58101 (+) 1380 WP_284173946.1 GspE/PulE family protein Machinery gene
  QMA14_RS00270 (QMA14_00270) pilC 58110..59333 (+) 1224 WP_049376124.1 type II secretion system F family protein Machinery gene
  QMA14_RS00275 (QMA14_00275) pilD 59330..60007 (+) 678 WP_049367123.1 A24 family peptidase Machinery gene
  QMA14_RS00280 (QMA14_00280) coaE 60094..60717 (+) 624 WP_005695506.1 dephospho-CoA kinase -
  QMA14_RS00285 (QMA14_00285) yacG 60707..60913 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  QMA14_RS00290 (QMA14_00290) - 60910..61191 (+) 282 WP_032822874.1 GNAT family N-acetyltransferase -
  QMA14_RS00295 (QMA14_00295) rhlB 61281..62540 (+) 1260 WP_115912279.1 ATP-dependent RNA helicase RhlB -
  QMA14_RS00300 (QMA14_00300) mreD 62581..63069 (-) 489 WP_005695501.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52181.57 Da        Isoelectric Point: 6.4484

>NTDB_id=829880 QMA14_RS00265 WP_284173946.1 56722..58101(+) (pilB) [Haemophilus parainfluenzae strain EL1]
MAYQAIAKNGEIYHISPQYWQQNQQQQALLLRYFTLPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETTQLK
QLLQSLAPKANQIEDQTTFYHHSEDENAANLSNEDEPVIQLLNSIFETALQKNASDIHLETQPSGLLIRLRIDGVLQPQP
VISKAFANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQK
QQIQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCGQHF
SDSCDCHQGYRGRIGVYQFLQCKNNRYQTDFDSLYQSALEKVKDHITDLDEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=829880 QMA14_RS00265 WP_284173946.1 56722..58101(+) (pilB) [Haemophilus parainfluenzae strain EL1]
ATGGCCTATCAAGCAATCGCAAAAAATGGCGAGATTTATCATATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCTTTACTGTTGCGTTACTTTACGCTCCCTCTTAAAGAAGACGAACACCATTTATGGCTTGCGGTGGATTCGCTAAATA
ATCTTGCAGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTAGTTGAACCGATTTTATTTGAAACGACTCAACTCAAA
CAACTTTTACAATCCCTTGCCCCTAAAGCCAATCAAATAGAAGACCAAACAACGTTTTATCATCATTCTGAAGACGAAAA
CGCTGCTAATTTATCTAATGAAGATGAACCCGTTATTCAATTATTAAATAGTATTTTTGAAACAGCCCTACAAAAAAATG
CCTCCGATATTCATTTAGAAACTCAGCCAAGTGGACTCTTAATTCGTTTACGTATTGATGGCGTACTACAGCCACAGCCG
GTAATCAGTAAAGCGTTCGCCAATCGTGTGATTTCTCGTTTAAAACTCTTAGCTAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGCTTTCAATTCAAAACCACGTTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACACATT
GGGGAGAAAAAGCGGTATTACGATTACAACAAAATAAACCAGTTCAGTTAAGTTTTGCTGAACTTGGCATGACACAAAAG
CAACAAATCCAATTTCAACACGCGCTCAGCCAACCTCAAGGTTTAATTCTTGTAACTGGACCAACAGGAAGCGGAAAAAG
TATCTCGCTTTATACCGCACTTCAATGGCTCAATACGCCAGATAAACATATTATGACAGCAGAAGATCCCGTTGAAATTG
AATTAGAAGGTATTATTCAAACTCAAGTGAATCCGCAAATCGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTACGA
CAAGATCCCGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTGATGGCATTAAGAGCCGCTCAAACCGG
CCATTTAGTGCTTTCTACCTTACATACCAACGATGCCATCTCAGCGATTTCACGCTTGCAACAACTAGGCATTCAATCTC
ACGAAATTGAAAACAGTCTCTTATTAGTTATCGCACAACGATTGGTGCGTAAACGTTGCTTAAAGTGCGGTCAACATTTC
AGTGATTCTTGCGATTGCCATCAGGGATACCGAGGTCGAATTGGGGTATATCAATTTTTACAATGCAAAAACAATCGCTA
CCAAACTGATTTCGATTCACTTTATCAAAGCGCGTTAGAAAAAGTAAAAGATCACATCACCGATCTCGATGAGATTCAAC
GCGTGTTAGGCAAAAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(124-391)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-88)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

77.876

98.475

0.767
  pilB Haemophilus influenzae Rd KW20

77.212

98.475

0.76
  pilB Glaesserella parasuis strain SC1401

59.071

98.475

0.582
  pilB Vibrio campbellii strain DS40M4

40.702

100

0.429
  pilB Vibrio cholerae strain A1552

40.496

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.417

100

0.423
  pilB Legionella pneumophila strain ERS1305867

38.855

100

0.414
  pilB Acinetobacter baumannii D1279779

38.604

100

0.41
  pilB Acinetobacter baylyi ADP1

37.827

100

0.41
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.383
  pilF Thermus thermophilus HB27

36.575

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.307

100

0.364