Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QMA14_RS00260 Genome accession   NZ_CP125087
Coordinates   56272..56715 (+) Length   147 a.a.
NCBI ID   WP_005697917.1    Uniprot ID   A0A369YN52
Organism   Haemophilus parainfluenzae strain EL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 51272..61715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMA14_RS00230 (QMA14_00230) tadA 51486..52004 (-) 519 WP_115912288.1 tRNA adenosine(34) deaminase TadA -
  QMA14_RS00235 (QMA14_00235) - 52014..52865 (-) 852 WP_115912286.1 thymidylate synthase -
  QMA14_RS00240 (QMA14_00240) lgt 52876..53685 (-) 810 WP_115912284.1 prolipoprotein diacylglyceryl transferase -
  QMA14_RS00245 (QMA14_00245) - 53694..54488 (-) 795 WP_005695514.1 sulfite exporter TauE/SafE family protein -
  QMA14_RS00250 (QMA14_00250) rppH 54488..55081 (-) 594 WP_049376123.1 RNA pyrophosphohydrolase -
  QMA14_RS00255 (QMA14_00255) ampD 55566..56129 (-) 564 WP_005695512.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QMA14_RS00260 (QMA14_00260) pilA 56272..56715 (+) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  QMA14_RS00265 (QMA14_00265) pilB 56722..58101 (+) 1380 WP_284173946.1 GspE/PulE family protein Machinery gene
  QMA14_RS00270 (QMA14_00270) pilC 58110..59333 (+) 1224 WP_049376124.1 type II secretion system F family protein Machinery gene
  QMA14_RS00275 (QMA14_00275) pilD 59330..60007 (+) 678 WP_049367123.1 A24 family peptidase Machinery gene
  QMA14_RS00280 (QMA14_00280) coaE 60094..60717 (+) 624 WP_005695506.1 dephospho-CoA kinase -
  QMA14_RS00285 (QMA14_00285) yacG 60707..60913 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  QMA14_RS00290 (QMA14_00290) - 60910..61191 (+) 282 WP_032822874.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15151.42 Da        Isoelectric Point: 8.4674

>NTDB_id=829879 QMA14_RS00260 WP_005697917.1 56272..56715(+) (pilA) [Haemophilus parainfluenzae strain EL1]
MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCVYSTNAPTNCSGGSNGIA
ADITTAKGYVKSITTKSGVITVTGNGALDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSPTK

Nucleotide


Download         Length: 444 bp        

>NTDB_id=829879 QMA14_RS00260 WP_005697917.1 56272..56715(+) (pilA) [Haemophilus parainfluenzae strain EL1]
ATGAAACTGACTTTTTCTAAACCTTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT
TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCGGCTATCTCCGAATTGTTACAAGCTTCGGCAC
CTTATAAATCTGATGTGGAGTTATGTGTCTATAGCACCAATGCCCCGACAAACTGTTCTGGTGGTTCAAATGGCATTGCA
GCAGACATCACTACTGCAAAAGGTTATGTTAAATCCATTACCACGAAATCGGGTGTGATTACGGTAACAGGAAATGGTGC
ATTAGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTTACCTGGACAACCGCTTGCCCAAGCA
ATGCGGATTTATTCCCTGCGGGTTTCTGCTCTCCTACCAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A369YN52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

75.694

97.959

0.741
  pilA Haemophilus influenzae Rd KW20

73.611

97.959

0.721
  pilA Glaesserella parasuis strain SC1401

56.081

100

0.565
  pilA Vibrio campbellii strain DS40M4

41.096

99.32

0.408
  pilE Neisseria gonorrhoeae MS11

41.985

89.116

0.374
  pilA2 Legionella pneumophila str. Paris

42.188

87.075

0.367