Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R8559_RS10380 Genome accession   NZ_AP026926
Coordinates   1963810..1964751 (-) Length   313 a.a.
NCBI ID   WP_000249559.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700119     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1958810..1969751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8559_RS10350 (PC0116_20210) - 1959058..1959333 (-) 276 WP_050202804.1 hypothetical protein -
  R8559_RS10355 (PC0116_20220) - 1959494..1960246 (+) 753 WP_219566337.1 XRE family transcriptional regulator -
  R8559_RS10360 (PC0116_20230) - 1960248..1961012 (+) 765 WP_219576745.1 hypothetical protein -
  R8559_RS10365 (PC0116_20240) - 1961319..1962764 (+) 1446 WP_317638087.1 recombinase family protein -
  R8559_RS10375 (PC0116_20250) comGB/cglB 1962846..1963862 (-) 1017 WP_073177425.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8559_RS10380 (PC0116_20260) comGA/cglA/cilD 1963810..1964751 (-) 942 WP_000249559.1 competence type IV pilus ATPase ComGA Machinery gene
  R8559_RS10385 (PC0116_20270) - 1964827..1965192 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8559_RS10390 (PC0116_20280) - 1965459..1966714 (+) 1256 WP_370657740.1 ISL3 family transposase -
  R8559_RS10395 (PC0116_20290) - 1966763..1967821 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R8559_RS10400 (PC0116_20300) nagA 1967984..1969135 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35489.42 Da        Isoelectric Point: 6.1829

>NTDB_id=82859 R8559_RS10380 WP_000249559.1 1963810..1964751(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700119]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHGLSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=82859 R8559_RS10380 WP_000249559.1 1963810..1964751(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700119]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGCTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGAAGTGGTAAGACGACCTTGATGCATGGATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGATATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.403

100

0.984
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comGA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comGA Streptococcus mutans UA159

66.238

99.361

0.658
  comGA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment