Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QJ050_RS13250 Genome accession   NZ_CP124840
Coordinates   2945011..2946120 (+) Length   369 a.a.
NCBI ID   WP_282789185.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain 29     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2940011..2951120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QJ050_RS13215 (QJ050_13215) - 2940600..2940899 (+) 300 WP_011899099.1 hypothetical protein -
  QJ050_RS13220 (QJ050_13220) - 2940968..2941387 (-) 420 WP_005320482.1 DUF4426 domain-containing protein -
  QJ050_RS13225 (QJ050_13225) yggU 2941406..2941705 (-) 300 WP_005320479.1 DUF167 family protein YggU -
  QJ050_RS13230 (QJ050_13230) - 2941705..2942256 (-) 552 WP_005320477.1 YggT family protein -
  QJ050_RS13235 (QJ050_13235) proC 2942285..2943109 (-) 825 WP_282789183.1 pyrroline-5-carboxylate reductase -
  QJ050_RS13240 (QJ050_13240) - 2943200..2943901 (-) 702 WP_282789184.1 YggS family pyridoxal phosphate-dependent enzyme -
  QJ050_RS13245 (QJ050_13245) pilT 2943941..2944975 (+) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  QJ050_RS13250 (QJ050_13250) pilU 2945011..2946120 (+) 1110 WP_282789185.1 type IVa pilus ATPase TapU Machinery gene
  QJ050_RS13255 (QJ050_13255) yaaA 2946152..2946925 (+) 774 WP_282789186.1 peroxide stress protein YaaA -
  QJ050_RS13260 (QJ050_13260) srmB 2947114..2948337 (-) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  QJ050_RS13265 (QJ050_13265) - 2948466..2949176 (+) 711 WP_096118856.1 methyltransferase -
  QJ050_RS13270 (QJ050_13270) brnQ 2949413..2950675 (+) 1263 WP_282789187.1 branched-chain amino acid transport system II carrier protein -
  QJ050_RS13275 (QJ050_13275) - 2950751..2950981 (-) 231 WP_192896245.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41097.10 Da        Isoelectric Point: 6.2347

>NTDB_id=828286 QJ050_RS13250 WP_282789185.1 2945011..2946120(+) (pilU) [Aeromonas salmonicida strain 29]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLVSLDSEALDKQSALTLVRETLSPEHFERYIRTREGNYAIHREALGR
FRVSAFWQQELPGMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=828286 QJ050_RS13250 WP_282789185.1 2945011..2946120(+) (pilU) [Aeromonas salmonicida strain 29]
ATGAATCTGGATGCCCTGTTGGCCGAACTGGTCGCACGCAAAGGCTCGGATCTTTTCATCACGGTTGGTACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGGTCTCCCTCGACAGCGAAGCGCTCGACAAGCAGAGCGCGCTGACCCTGGTCAGGGAAA
CGCTCAGCCCTGAGCATTTCGAGCGATACATCCGCACTAGGGAGGGCAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTCGGGTGAGTGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGGTGCGCCGTATCGAGACCCGCATTCCAACCTTCGA
CGAACTGACCCTGCCGCCCATCTTGCAGGAGGTGGCCATGGCCAAACGCGGTCTGGTGCTGTTCGTCGGCGCCACCGGAA
CGGGCAAGTCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGATGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGGGAAGTGGGCATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCACCTGACGTTATCCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGTCATCTTTGCCTAGCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAACACCGCCAGTTACTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGTCGCCGCCGCTTCGCGGCCTTTGAAGTGCTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGTCTCAAGGAGGTGATGACCAAATCCACCGAGCTTGGTATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.239

99.729

0.591
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36