Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QK281_RS03440 Genome accession   NZ_CP124746
Coordinates   702263..703372 (-) Length   369 a.a.
NCBI ID   WP_282467215.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain NN-MR659     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697263..708372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QK281_RS03420 (QK281_03420) brnQ 697720..699027 (-) 1308 WP_282467212.1 branched-chain amino acid transport system II carrier protein -
  QK281_RS03425 (QK281_03425) - 699219..699929 (-) 711 WP_282467213.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  QK281_RS03430 (QK281_03430) srmB 700058..701281 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  QK281_RS03435 (QK281_03435) yaaA 701458..702231 (-) 774 WP_282467214.1 peroxide stress protein YaaA -
  QK281_RS03440 (QK281_03440) pilU 702263..703372 (-) 1110 WP_282467215.1 type IVa pilus ATPase TapU Machinery gene
  QK281_RS03445 (QK281_03445) pilT 703401..704435 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  QK281_RS03450 (QK281_03450) - 704475..705176 (+) 702 WP_282467216.1 YggS family pyridoxal phosphate-dependent enzyme -
  QK281_RS03455 (QK281_03455) proC 705235..706059 (+) 825 WP_041216516.1 pyrroline-5-carboxylate reductase -
  QK281_RS03460 (QK281_03460) - 706100..706651 (+) 552 WP_282467217.1 YggT family protein -
  QK281_RS03465 (QK281_03465) yggU 706651..706950 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  QK281_RS03470 (QK281_03470) - 706969..707388 (+) 420 WP_202004565.1 DUF4426 domain-containing protein -
  QK281_RS03475 (QK281_03475) - 707443..707754 (-) 312 WP_219281984.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41060.17 Da        Isoelectric Point: 6.4493

>NTDB_id=827895 QK281_RS03440 WP_282467215.1 702263..703372(-) (pilU) [Aeromonas hydrophila strain NN-MR659]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALTLVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGHRRCAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=827895 QK281_RS03440 WP_282467215.1 702263..703372(-) (pilU) [Aeromonas hydrophila strain NN-MR659]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCGCTGGCAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGACGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCACACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAACTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGTTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAAAGCTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGTGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACTGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGGCACCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363