Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   P7I81_RS05800 Genome accession   NZ_CP124648
Coordinates   1222035..1223252 (+) Length   405 a.a.
NCBI ID   WP_003107299.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2020CK-00220     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1217035..1228252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I81_RS05775 (P7I81_05775) nadC 1217156..1218004 (+) 849 WP_124089605.1 carboxylating nicotinate-nucleotide diphosphorylase -
  P7I81_RS05785 (P7I81_05785) - 1218185..1219570 (-) 1386 WP_074222679.1 O-antigen ligase family protein -
  P7I81_RS05790 (P7I81_05790) - 1219636..1220100 (-) 465 WP_124089607.1 pilin -
  P7I81_RS05795 (P7I81_05795) pilB 1220331..1222031 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P7I81_RS05800 (P7I81_05800) pilC 1222035..1223252 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  P7I81_RS05805 (P7I81_05805) pilD 1223253..1224125 (+) 873 WP_023084852.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  P7I81_RS05810 (P7I81_05810) coaE 1224122..1224733 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  P7I81_RS05815 (P7I81_05815) yacG 1224730..1224930 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  P7I81_RS05820 (P7I81_05820) - 1224967..1225176 (-) 210 WP_003094660.1 hypothetical protein -
  P7I81_RS05825 (P7I81_05825) - 1225282..1225971 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  P7I81_RS05830 (P7I81_05830) - 1225968..1226438 (-) 471 WP_003094664.1 hypothetical protein -
  P7I81_RS05835 (P7I81_05835) - 1226435..1226860 (-) 426 WP_124089609.1 GNAT family N-acetyltransferase -
  P7I81_RS05840 (P7I81_05840) - 1226993..1227622 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  P7I81_RS05845 (P7I81_05845) - 1227619..1228068 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44001.86 Da        Isoelectric Point: 9.8103

>NTDB_id=827034 P7I81_RS05800 WP_003107299.1 1222035..1223252(+) (pilC) [Pseudomonas aeruginosa strain 2020CK-00220]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=827034 P7I81_RS05800 WP_003107299.1 1222035..1223252(+) (pilC) [Pseudomonas aeruginosa strain 2020CK-00220]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGG
TCATCAACATTTCCAACGTACTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTTTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

61.029

100

0.615
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

46.04

99.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Vibrio campbellii strain DS40M4

40.648

99.012

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378