Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   R6U47_RS27290 Genome accession   NZ_CP137561
Coordinates   5805716..5807860 (+) Length   714 a.a.
NCBI ID   WP_324001566.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CPA0053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5800716..5812860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6U47_RS27270 (R6U47_27275) pilM 5802861..5803925 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  R6U47_RS27275 (R6U47_27280) pilN 5803925..5804521 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  R6U47_RS27280 (R6U47_27285) pilO 5804518..5805141 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  R6U47_RS27285 (R6U47_27290) comL/pilP 5805138..5805662 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  R6U47_RS27290 (R6U47_27295) pilQ 5805716..5807860 (+) 2145 WP_324001566.1 type 4a pilus secretin PilQ Machinery gene
  R6U47_RS27295 (R6U47_27300) aroK 5807872..5808390 (+) 519 WP_003095833.1 shikimate kinase AroK -
  R6U47_RS27300 (R6U47_27305) aroB 5808440..5809546 (+) 1107 WP_003107839.1 3-dehydroquinate synthase -
  R6U47_RS27305 (R6U47_27310) - 5809553..5811208 (+) 1656 WP_003141778.1 AAA family ATPase -

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77438.00 Da        Isoelectric Point: 5.3239

>NTDB_id=822503 R6U47_RS27290 WP_324001566.1 5805716..5807860(+) (pilQ) [Pseudomonas aeruginosa strain CPA0053]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTALSSYTTRVEGNNLFVVVGNSPTGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
SGYGKDGNIGIKDEDGMNCGPIAGSCTFPTTGTSKSPSPFVDLGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNNGETIVIGGVFSNEQSKSVEKVPFLGELPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=822503 R6U47_RS27290 WP_324001566.1 5805716..5807860(+) (pilQ) [Pseudomonas aeruginosa strain CPA0053]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCGCTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCGCGCT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGACCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAACCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGATGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAACTGATCGCCGACTTCACCGACCTGAATCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTACTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTACTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGCCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGTCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AGCGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGTAGCTGTAC
CTTCCCGACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACCTCGGTGCCAAGGATGCTACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAACCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCTCGGAAGTCCCCTACCAGGAGGCCAGTTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAAGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTGAAGGTGACCAAGGACGCGCCGGATTACCAGAATATGTTGAACGGCGTACCTCCTATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACAACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGTAACGAGCAGAGCAAGTC
GGTGGAGAAGGTGCCGTTCCTCGGCGAACTGCCTTACCTGGGGCGGCTGTTCCGGCGTGATACCGTCACCGACCGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterProScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(549-705)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

99.58

100

0.996
  pilQ Acinetobacter baumannii D1279779

40.839

100

0.409
  pilQ Acinetobacter baumannii strain A118

40.839

100

0.409
  comQ/pilQ Acinetobacter baylyi ADP1

41.311

98.319

0.406
  pilQ Legionella pneumophila strain Lp02

38.248

95.938

0.367
  pilQ Legionella pneumophila strain ERS1305867

38.102

95.938

0.366