Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   R6U47_RS27280 Genome accession   NZ_CP137561
Coordinates   5804518..5805141 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain CPA0053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5799518..5810141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6U47_RS27265 (R6U47_27270) - 5800205..5802676 (-) 2472 WP_016562439.1 penicillin-binding protein 1A -
  R6U47_RS27270 (R6U47_27275) pilM 5802861..5803925 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  R6U47_RS27275 (R6U47_27280) pilN 5803925..5804521 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  R6U47_RS27280 (R6U47_27285) pilO 5804518..5805141 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  R6U47_RS27285 (R6U47_27290) comL/pilP 5805138..5805662 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  R6U47_RS27290 (R6U47_27295) pilQ 5805716..5807860 (+) 2145 WP_324001566.1 type 4a pilus secretin PilQ Machinery gene
  R6U47_RS27295 (R6U47_27300) aroK 5807872..5808390 (+) 519 WP_003095833.1 shikimate kinase AroK -
  R6U47_RS27300 (R6U47_27305) aroB 5808440..5809546 (+) 1107 WP_003107839.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=822501 R6U47_RS27280 WP_003103268.1 5804518..5805141(+) (pilO) [Pseudomonas aeruginosa strain CPA0053]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=822501 R6U47_RS27280 WP_003103268.1 5804518..5805141(+) (pilO) [Pseudomonas aeruginosa strain CPA0053]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGTGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAATGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401