Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   QEP71_RS02025 Genome accession   NZ_CP123204
Coordinates   429611..430828 (-) Length   405 a.a.
NCBI ID   WP_005732330.1    Uniprot ID   A0A3M4TFK7
Organism   Pseudomonas amygdali pv. aesculi strain 6617     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424611..435828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEP71_RS01990 - 424645..425943 (-) 1299 WP_005732325.1 NAD(P)/FAD-dependent oxidoreductase -
  QEP71_RS29655 - 426040..426276 (-) 237 WP_327203937.1 DUF3094 family protein -
  QEP71_RS01995 - 426161..426694 (-) 534 WP_005732326.1 MOSC domain-containing protein -
  QEP71_RS02000 - 426691..427320 (-) 630 WP_003430735.1 DUF1780 domain-containing protein -
  QEP71_RS02005 - 427462..427713 (+) 252 Protein_389 hypothetical protein -
  QEP71_RS02010 yacG 427911..428120 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  QEP71_RS02015 coaE 428117..428740 (-) 624 WP_005732328.1 dephospho-CoA kinase -
  QEP71_RS02020 pilD 428737..429609 (-) 873 WP_010216946.1 A24 family peptidase Machinery gene
  QEP71_RS02025 pilC 429611..430828 (-) 1218 WP_005732330.1 type II secretion system F family protein Machinery gene
  QEP71_RS02030 pilB 430831..432525 (-) 1695 WP_010216952.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QEP71_RS02035 - 432753..433181 (+) 429 WP_005732335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QEP71_RS02040 - 434014..434559 (-) 546 WP_010216954.1 DUF1523 family protein -
  QEP71_RS02045 - 434679..435677 (+) 999 WP_005732338.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44117.07 Da        Isoelectric Point: 10.2266

>NTDB_id=819979 QEP71_RS02025 WP_005732330.1 429611..430828(-) (pilC) [Pseudomonas amygdali pv. aesculi strain 6617]
MASKAAKVIVYTWEGVDKKGTKTSGELNGHNLALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRSLVESLKQEVSAGNSFATALRRKPEYFDELFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAIIVSGILLIKVVPQFQSIFSSFGAELPAFTLMVIGLSDIVQKWWLAIVFAFFSGIFALK
RVYKKSQKFRDGLDRLLLKVPIIGPLIFKSSVARYARTLSTTFAAGVPLVEALDSVAGATGNVVFKNAVQKVKQDVSTGM
QLNFSMRSTGVFPTLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=819979 QEP71_RS02025 WP_005732330.1 429611..430828(-) (pilC) [Pseudomonas amygdali pv. aesculi strain 6617]
ATGGCCAGCAAGGCAGCAAAAGTCATCGTTTACACTTGGGAAGGTGTCGACAAGAAAGGCACCAAGACCAGCGGCGAACT
GAACGGCCATAACCTGGCACTCGTAAAAGCCCAGTTGCGCAAGCAAGGTATCAACCCCACCAAGGTACGCAAAAAATCTG
CGTCGATCTTCGGCAAAGGCAAGAAGATTAAGCCATTGGACATCGCGTTTTTTTCTCGGCAGATGGCAACGATGATGAAG
GCTGGCGTGCCATTGCTGCAGTCGTTTGACATCATCAGTGAGGGTGCTGAAAATCCGAATATGCGGTCGCTGGTAGAATC
TCTAAAGCAGGAGGTTTCAGCCGGCAACAGCTTTGCCACCGCGTTACGAAGAAAACCAGAATACTTCGATGAGCTTTTCT
GTAATTTAGTGGATGCAGGGGAACAGGCGGGAGCACTTGAAAGCCTGCTGGACCGAGTAGCAAGCTACAAGGAGAAGACA
GAGAAGCTCAAGGCTAAGATAAAAAAGGCCATGACCTACCCGGCCGCAGTATTGATTGTGGCGATCATCGTTTCAGGTAT
TTTGCTTATAAAGGTTGTGCCGCAGTTTCAGTCAATTTTTTCCAGCTTTGGTGCGGAACTACCGGCCTTTACGTTAATGG
TTATCGGTCTTTCAGACATAGTACAAAAGTGGTGGCTAGCTATCGTATTCGCTTTTTTTTCTGGCATTTTTGCACTTAAA
CGAGTATACAAAAAATCTCAAAAATTTCGTGACGGACTTGATCGTTTATTATTAAAAGTACCGATAATTGGCCCGTTAAT
TTTTAAATCCTCGGTCGCACGATACGCCCGAACCCTTTCAACTACGTTCGCCGCTGGTGTTCCGCTGGTAGAGGCTCTTG
ATTCGGTCGCTGGGGCCACTGGCAACGTAGTCTTCAAAAACGCGGTCCAAAAAGTCAAACAGGACGTTTCCACTGGTATG
CAGTTGAATTTCTCGATGCGTTCGACAGGTGTGTTCCCTACCTTAGCGATCCAAATGACCGCAATTGGTGAGGAATCAGG
CGCGTTAGACACCATGCTGGACAAGGTAGCGACCTACTACGAGGATGAGGTCGACAATATGGTCGACAACCTAACCAGCC
TGATGGAGCCCATGATCATGGCTGTTCTGGGTGTCATCGTCGGCGGCCTTGTTATCGCCATGTACCTCCCCATTTTCAAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M4TFK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.086

100

0.731
  pilC Acinetobacter baylyi ADP1

60.049

100

0.605
  pilC Acinetobacter baumannii D1279779

59.804

100

0.602
  pilC Legionella pneumophila strain ERS1305867

51.232

100

0.514
  pilC Vibrio campbellii strain DS40M4

42.365

100

0.425
  pilC Vibrio cholerae strain A1552

42.929

97.778

0.42
  pilG Neisseria gonorrhoeae MS11

42.065

98.025

0.412
  pilG Neisseria meningitidis 44/76-A

42.065

98.025

0.412
  pilC Thermus thermophilus HB27

38.404

99.012

0.38