Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R4535_RS10680 Genome accession   NZ_CP137091
Coordinates   2384421..2384825 (+) Length   134 a.a.
NCBI ID   WP_057551631.1    Uniprot ID   -
Organism   Vibrio cholerae strain DL4211     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2379421..2389825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4535_RS10660 (R4535_10635) fldB 2380355..2380873 (+) 519 WP_000690112.1 flavodoxin FldB -
  R4535_RS10665 (R4535_10640) - 2380920..2382308 (-) 1389 WP_407284727.1 IS4-like element ISVa18 family transposase -
  R4535_RS10670 (R4535_10645) ampD 2382445..2382990 (-) 546 WP_076025708.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R4535_RS10675 (R4535_10650) nadC 2383286..2384176 (+) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R4535_RS10680 (R4535_10655) pilA 2384421..2384825 (+) 405 WP_057551631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  R4535_RS10685 (R4535_10660) pilB 2384832..2386520 (+) 1689 WP_076025706.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R4535_RS10690 (R4535_10665) pilC 2386563..2387789 (+) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  R4535_RS10695 (R4535_10670) pilD 2387847..2388722 (+) 876 WP_076025705.1 A24 family peptidase Machinery gene
  R4535_RS10700 (R4535_10675) coaE 2388719..2389327 (+) 609 WP_175597578.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13586.69 Da        Isoelectric Point: 9.0173

>NTDB_id=818244 R4535_RS10680 WP_057551631.1 2384421..2384825(+) (pilA) [Vibrio cholerae strain DL4211]
MKAYKNKLQQGFTLIELMIVVAVIGVLAAIAVPQYQKYTQKAALATALATATALKTNYEDYVSMSGAAPTSATDIGATSF
SIGTISVTSGAINVAINKGPGTGSSVILTRTGLLGGWGCQISGAQAEVTINGCP

Nucleotide


Download         Length: 405 bp        

>NTDB_id=818244 R4535_RS10680 WP_057551631.1 2384421..2384825(+) (pilA) [Vibrio cholerae strain DL4211]
ATGAAAGCGTATAAAAATAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTAGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCGGTGCCTCAATATCAGAAATATACACAAAAAGCAGCTCTTGCTACTGCTTTAGCTACAGCAACTGCAT
TAAAAACAAATTATGAAGACTATGTATCTATGAGTGGTGCAGCTCCCACATCTGCAACAGATATTGGAGCAACCAGCTTC
AGTATAGGAACTATTTCAGTGACTAGTGGTGCTATAAACGTTGCTATAAATAAGGGACCAGGCACTGGCTCATCTGTAAT
TTTAACTAGAACAGGGCTTCTTGGTGGCTGGGGATGCCAAATCTCCGGAGCTCAAGCTGAAGTAACAATTAACGGTTGCC
CATAG

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.243

100

0.478
  pilA Vibrio cholerae strain A1552

43.243

100

0.478
  pilA Vibrio cholerae C6706

43.243

100

0.478
  pilA Vibrio parahaemolyticus RIMD 2210633

39.189

100

0.433
  pilA Pseudomonas aeruginosa PAK

38.514

100

0.425
  pilA Vibrio campbellii strain DS40M4

37.013

100

0.425
  pilA Acinetobacter baumannii strain A118

38.519

100

0.388
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.462

97.015

0.373
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.2

93.284

0.366