Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS37_RS02530 Genome accession   NZ_CP121845
Coordinates   485827..486936 (-) Length   369 a.a.
NCBI ID   WP_129504783.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 15694     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480827..491936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS37_RS02500 - 481279..482286 (-) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -
  QBS37_RS02505 glnK 482545..482883 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS37_RS02510 arfB 483085..483501 (+) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS37_RS02515 - 483534..484025 (+) 492 WP_201914207.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS37_RS02520 - 484386..484811 (-) 426 WP_005340360.1 hypothetical protein -
  QBS37_RS02525 yaaA 485006..485779 (-) 774 WP_005340358.1 peroxide stress protein YaaA -
  QBS37_RS02530 pilU 485827..486936 (-) 1110 WP_129504783.1 type IVa pilus ATPase TapU Machinery gene
  QBS37_RS02535 pilT 486965..487999 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS37_RS02540 - 488039..488740 (+) 702 WP_139441948.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS37_RS02545 proC 488883..489707 (+) 825 WP_139441947.1 pyrroline-5-carboxylate reductase -
  QBS37_RS02550 - 489719..490270 (+) 552 WP_047436998.1 YggT family protein -
  QBS37_RS02555 yggU 490270..490572 (+) 303 WP_005340341.1 DUF167 family protein YggU -
  QBS37_RS02560 - 490616..491035 (+) 420 WP_033135536.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41075.13 Da        Isoelectric Point: 6.6380

>NTDB_id=814651 QBS37_RS02530 WP_129504783.1 485827..486936(-) (pilU) [Aeromonas veronii strain ANYA 15694]
MNLDDLLSQLVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814651 QBS37_RS02530 WP_129504783.1 485827..486936(-) (pilU) [Aeromonas veronii strain ANYA 15694]
ATGAATCTGGATGATCTGCTGAGCCAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCTCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATTTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCATTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTTCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAATTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTTGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TTCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACTGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

55.858

99.458

0.556
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36