Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS27_RS17090 Genome accession   NZ_CP121840
Coordinates   3707740..3708849 (-) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18197     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3702740..3713849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS27_RS17060 - 3703192..3704199 (-) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -
  QBS27_RS17065 glnK 3704458..3704796 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS27_RS17070 arfB 3704998..3705414 (+) 417 WP_279489791.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS27_RS17075 - 3705447..3705938 (+) 492 WP_279489792.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS27_RS17080 - 3706299..3706724 (-) 426 WP_108613288.1 hypothetical protein -
  QBS27_RS17085 yaaA 3706919..3707692 (-) 774 WP_120414752.1 peroxide stress protein YaaA -
  QBS27_RS17090 pilU 3707740..3708849 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  QBS27_RS17095 pilT 3708878..3709912 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS27_RS17100 - 3709952..3710653 (+) 702 WP_019445603.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS27_RS17105 proC 3710796..3711620 (+) 825 WP_279489793.1 pyrroline-5-carboxylate reductase -
  QBS27_RS17110 - 3711632..3712183 (+) 552 WP_005340343.1 YggT family protein -
  QBS27_RS17115 yggU 3712183..3712485 (+) 303 WP_005340341.1 DUF167 family protein YggU -
  QBS27_RS17120 - 3712529..3712948 (+) 420 WP_279489794.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=814559 QBS27_RS17090 WP_005340356.1 3707740..3708849(-) (pilU) [Aeromonas veronii strain ANYA 18197]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814559 QBS27_RS17090 WP_005340356.1 3707740..3708849(-) (pilU) [Aeromonas veronii strain ANYA 18197]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGTGGGGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGTTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACTTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCAGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTTCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAATTCGCCGAGACAGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATTCTGCTCAATACTCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGTTGGGTATGCAGACCTTCGATCAGGCG
CTTTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36