Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS19_RS07825 Genome accession   NZ_CP121832
Coordinates   1505494..1506603 (+) Length   369 a.a.
NCBI ID   WP_279460526.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18661     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1500494..1511603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS19_RS07795 - 1501395..1501814 (-) 420 WP_279460390.1 DUF4426 domain-containing protein -
  QBS19_RS07800 yggU 1501858..1502160 (-) 303 WP_279460391.1 DUF167 family protein YggU -
  QBS19_RS07805 - 1502160..1502711 (-) 552 WP_005340343.1 YggT family protein -
  QBS19_RS07810 proC 1502723..1503547 (-) 825 WP_279460392.1 pyrroline-5-carboxylate reductase -
  QBS19_RS07815 - 1503690..1504391 (-) 702 WP_151286205.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS19_RS07820 pilT 1504431..1505465 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS19_RS07825 pilU 1505494..1506603 (+) 1110 WP_279460526.1 type IVa pilus ATPase TapU Machinery gene
  QBS19_RS07830 yaaA 1506651..1507424 (+) 774 WP_005340358.1 peroxide stress protein YaaA -
  QBS19_RS07835 - 1507619..1508044 (+) 426 WP_005340360.1 hypothetical protein -
  QBS19_RS07840 - 1508405..1508896 (-) 492 WP_201914207.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS19_RS07845 arfB 1508929..1509345 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS19_RS07850 glnK 1509547..1509885 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS19_RS07855 - 1510144..1511151 (+) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41061.10 Da        Isoelectric Point: 6.6379

>NTDB_id=814445 QBS19_RS07825 WP_279460526.1 1505494..1506603(+) (pilU) [Aeromonas veronii strain ANYA 18661]
MNLDDLLSQLVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814445 QBS19_RS07825 WP_279460526.1 1505494..1506603(+) (pilU) [Aeromonas veronii strain ANYA 18661]
ATGAATCTGGATGATCTGCTGAGCCAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCTCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATTTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCATTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGATGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTTCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAATTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTTGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TTCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACTGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

55.586

99.458

0.553
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.118

91.87

0.369
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36