Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS22_RS07820 Genome accession   NZ_CP121801
Coordinates   1619588..1620697 (-) Length   369 a.a.
NCBI ID   WP_010636179.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BOND 19077     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1614588..1625697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS22_RS07800 brnQ 1615046..1616353 (-) 1308 WP_005305030.1 branched-chain amino acid transport system II carrier protein -
  QBS22_RS07805 - 1616544..1617254 (-) 711 WP_042007904.1 methyltransferase -
  QBS22_RS07810 srmB 1617383..1618606 (+) 1224 WP_279485266.1 ATP-dependent RNA helicase SrmB -
  QBS22_RS07815 yaaA 1618783..1619556 (-) 774 WP_010636178.1 peroxide stress protein YaaA -
  QBS22_RS07820 pilU 1619588..1620697 (-) 1110 WP_010636179.1 type IVa pilus ATPase TapU Machinery gene
  QBS22_RS07825 pilT 1620726..1621760 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  QBS22_RS07830 - 1621800..1622501 (+) 702 WP_279462358.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS22_RS07835 proC 1622562..1623386 (+) 825 WP_279485267.1 pyrroline-5-carboxylate reductase -
  QBS22_RS07840 - 1623427..1623978 (+) 552 WP_279485268.1 YggT family protein -
  QBS22_RS07845 yggU 1623978..1624277 (+) 300 WP_005304998.1 DUF167 family protein YggU -
  QBS22_RS07850 - 1624296..1624715 (+) 420 WP_005304995.1 DUF4426 domain-containing protein -
  QBS22_RS07855 - 1624779..1625090 (-) 312 WP_279462357.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41081.22 Da        Isoelectric Point: 6.5672

>NTDB_id=814339 QBS22_RS07820 WP_010636179.1 1619588..1620697(-) (pilU) [Aeromonas dhakensis strain BOND 19077]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814339 QBS22_RS07820 WP_010636179.1 1619588..1620697(-) (pilU) [Aeromonas dhakensis strain BOND 19077]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCGCCACTCGACAAGGCAAGGGCACTGGCACTGGTGAAGGAGA
CCCTGAGCCCGGAGCACTTCGAACGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTACTGCGCCGCATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATATTGCAGGAGATCGCCCTCGCCAAGCGCGGACTTGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGCTCACTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTGCACCTGGTGCCCCAGGAGAAACACCGTCAGTTCCTGTTTGATCTCTCCTTCAACCTGCGCGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAACAGCTGGGTTCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363