Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS05_RS00795 Genome accession   NZ_CP121795
Coordinates   145422..146531 (+) Length   369 a.a.
NCBI ID   WP_010636179.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain DAMIAN 12594     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 140422..151531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS05_RS00760 - 141029..141340 (+) 312 WP_279462357.1 hypothetical protein -
  QBS05_RS00765 - 141404..141823 (-) 420 WP_005304995.1 DUF4426 domain-containing protein -
  QBS05_RS00770 yggU 141842..142141 (-) 300 WP_005304998.1 DUF167 family protein YggU -
  QBS05_RS00775 - 142141..142692 (-) 552 WP_017780458.1 YggT family protein -
  QBS05_RS00780 proC 142733..143557 (-) 825 WP_017783067.1 pyrroline-5-carboxylate reductase -
  QBS05_RS00785 - 143618..144319 (-) 702 WP_279462358.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS05_RS00790 pilT 144359..145393 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  QBS05_RS00795 pilU 145422..146531 (+) 1110 WP_010636179.1 type IVa pilus ATPase TapU Machinery gene
  QBS05_RS00800 yaaA 146563..147336 (+) 774 WP_279462359.1 peroxide stress protein YaaA -
  QBS05_RS00805 srmB 147513..148736 (-) 1224 WP_279462360.1 ATP-dependent RNA helicase SrmB -
  QBS05_RS00810 - 148865..149575 (+) 711 WP_117405566.1 methyltransferase -
  QBS05_RS00815 brnQ 149767..151074 (+) 1308 WP_005305030.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41081.22 Da        Isoelectric Point: 6.5672

>NTDB_id=814244 QBS05_RS00795 WP_010636179.1 145422..146531(+) (pilU) [Aeromonas dhakensis strain DAMIAN 12594]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814244 QBS05_RS00795 WP_010636179.1 145422..146531(+) (pilU) [Aeromonas dhakensis strain DAMIAN 12594]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCGCCACTCGACAAGGCAAGGGCACTGGCACTGGTGAAGGAGA
CCCTGAGCCCGGAGCACTTCGAACGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTACTGCGCCGCATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATATTGCAGGAGATCGCCCTCGCCAAGCGCGGACTTGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGCTCACTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTGCACCTGGTGCCCCAGGAGAAACACCGTCAGTTCCTGTTTGATCTCTCCTTCAACCTGCGCGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAACAGCTGGGTTCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363