Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   RQ453_RS16930 Genome accession   NZ_CP135173
Coordinates   3609908..3610372 (+) Length   154 a.a.
NCBI ID   WP_023092872.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CP-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3604908..3615372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RQ453_RS16905 (RQ453_16905) yacG 3605078..3605278 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  RQ453_RS16910 (RQ453_16910) coaE 3605275..3605886 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  RQ453_RS16915 (RQ453_16915) pilD 3605883..3606755 (-) 873 WP_023092875.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  RQ453_RS16920 (RQ453_16920) pilC 3606756..3607973 (-) 1218 WP_033995372.1 type II secretion system F family protein Machinery gene
  RQ453_RS16925 (RQ453_16925) pilB 3607977..3609677 (-) 1701 WP_023092873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RQ453_RS16930 (RQ453_16930) pilA/pilAI 3609908..3610372 (+) 465 WP_023092872.1 pilin Machinery gene
  RQ453_RS16935 (RQ453_16935) - 3610528..3611841 (+) 1314 WP_231280937.1 O-antigen ligase family protein -
  RQ453_RS16945 (RQ453_16945) nadC 3612022..3612870 (-) 849 WP_033995373.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16090.43 Da        Isoelectric Point: 8.9912

>NTDB_id=810280 RQ453_RS16930 WP_023092872.1 3609908..3610372(+) (pilA/pilAI) [Pseudomonas aeruginosa strain CP-1]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGGSSGKGIAGAVITVKRDDKGVWTCGITGKPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=810280 RQ453_RS16930 WP_023092872.1 3609908..3610372(+) (pilA/pilAI) [Pseudomonas aeruginosa strain CP-1]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGTAAGAAGCTCGTTTCTAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCGGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGAAAGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.161

100

0.455
  pilA Pseudomonas aeruginosa PAK

41.772

100

0.429
  pilA Acinetobacter baumannii strain A118

45.07

92.208

0.416
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364
  pilA2 Legionella pneumophila strain ERS1305867

37.838

96.104

0.364