Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RO839_RS10815 Genome accession   NZ_CP135132
Coordinates   2369953..2370399 (+) Length   148 a.a.
NCBI ID   WP_057682388.1    Uniprot ID   -
Organism   Xylella fastidiosa strain CO33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2364953..2375399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RO839_RS10795 (RO839_10795) - 2365201..2366538 (+) 1338 WP_057682389.1 HAMP domain-containing sensor histidine kinase -
  RO839_RS10800 (RO839_10800) coaE 2366805..2367428 (-) 624 WP_020851236.1 dephospho-CoA kinase -
  RO839_RS10805 (RO839_10805) pilD 2367440..2368303 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  RO839_RS10810 (RO839_10810) pilC 2368310..2369512 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  RO839_RS10815 (RO839_10815) pilA 2369953..2370399 (+) 447 WP_057682388.1 pilin Machinery gene
  RO839_RS10820 (RO839_10820) - 2370645..2370872 (-) 228 WP_057682387.1 hypothetical protein -
  RO839_RS10825 (RO839_10825) - 2371260..2371562 (-) 303 WP_128734940.1 hypothetical protein -
  RO839_RS10830 (RO839_10830) pilA/pilAI 2371706..2372140 (+) 435 WP_004084597.1 pilin Machinery gene
  RO839_RS10835 (RO839_10835) pilB 2372980..2374713 (+) 1734 WP_057682386.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15372.58 Da        Isoelectric Point: 8.4988

>NTDB_id=810146 RO839_RS10815 WP_057682388.1 2369953..2370399(+) (pilA) [Xylella fastidiosa strain CO33]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKATSNASDIGLRTDTTRCGI
TVKVEAAGTANITCKIKGNSQVSDKTIAWDRTPDNSAGTNGVNNGGVWTCSTTVTSDALRPSGCIASK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=810146 RO839_RS10815 WP_057682388.1 2369953..2370399(+) (pilA) [Xylella fastidiosa strain CO33]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTTCTGGCGGCCATCGCTCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACTGCGGCACTTGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGGCTACATCCAACGCGAGTGATATCGGTCTGCGTACTGACACCACTCGTTGTGGAATT
ACCGTTAAAGTCGAGGCCGCTGGCACTGCCAACATCACATGCAAAATTAAGGGCAACTCTCAAGTCAGTGATAAAACCAT
CGCTTGGGATCGTACCCCCGATAACTCTGCAGGCACCAATGGCGTCAATAATGGAGGTGTGTGGACCTGCTCCACCACCG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATTGCCTCTAAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40

100

0.419
  pilA Vibrio cholerae strain A1552

40

100

0.419
  pilA Vibrio cholerae C6706

40

100

0.419
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.497

99.324

0.412
  pilA Pseudomonas aeruginosa PAK

37.736

100

0.405
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.816

99.324

0.405
  pilA Acinetobacter baumannii strain A118

38

100

0.385
  pilA Vibrio parahaemolyticus RIMD 2210633

36.486

100

0.365