Detailed information NTG0805792

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUC147_RS08960
• Genome accession: NZ_CP120473
• Coordinates: 1788487..1789068 (-)
• Length: 193 a.a.
• NCBI ID: WP_231097919.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain UC147
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1783487..1794068

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC147_RS08935 (LLUC147_08975)	-	1784209..1784514 (-)	306	Protein_1741	ComEC/Rec2 family competence protein	-
LLUC147_RS08940 (LLUC147_08980)	-	1784535..1785425 (-)	891	WP_281634794.1	IS982-like element IS982B family transposase	-
LLUC147_RS08945 (LLUC147_08985)	comEC	1785502..1786011 (-)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
LLUC147_RS08950 (LLUC147_08990)	-	1786322..1787269 (+)	948	WP_003130410.1	IS30 family transposase	-
LLUC147_RS08955 (LLUC147_08995)	comEC	1787229..1788506 (-)	1278	WP_396425195.1	ComEC/Rec2 family competence protein	Machinery gene
LLUC147_RS08960 (LLUC147_09000)	comEA	1788487..1789068 (-)	582	WP_231097919.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLUC147_RS08965 (LLUC147_09005)	-	1789199..1790560 (-)	1362	WP_081196831.1	ABC transporter permease	-
LLUC147_RS08970 (LLUC147_09010)	-	1790557..1791489 (-)	933	WP_014572202.1	ABC transporter ATP-binding protein	-
LLUC147_RS08975 (LLUC147_09015)	-	1791595..1791993 (-)	399	WP_011676775.1	hypothetical protein	-
LLUC147_RS08980 (LLUC147_09020)	-	1792085..1792669 (-)	585	WP_134047437.1	GNAT family N-acetyltransferase	-
LLUC147_RS08985 (LLUC147_09025)	-	1792842..1794020 (-)	1179	WP_011676777.1	SLC13 family permease	-

Sequence

Protein

• Length: 193 a.a.
• Molecular weight: 21052.51 Da
• Isoelectric Point: 4.8079

>NTDB_id=805792 LLUC147_RS08960 WP_231097919.1 1788487..1789068(-) (comEA) [Lactococcus cremoris strain UC147]
MEFLYIFTNSPKQAENLSVENLSSSSTKSSVSKFNSSSEKNKNEIMVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQ
ADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVSTTSGPKININKADLTELQKLTGIGQKKAQDIIDFR
MKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 582 bp

>NTDB_id=805792 LLUC147_RS08960 WP_231097919.1 1788487..1789068(-) (comEA) [Lactococcus cremoris strain UC147]
GTGGAATTTTTATATATTTTCACCAATTCACCAAAGCAAGCTGAAAATCTGTCAGTAGAAAATTTGAGCTCTAGTTCTAC
CAAAAGCTCTGTCAGTAAATTTAATAGCAGCAGTGAAAAAAATAAAAACGAAATTATGGTTGATTTGAAAGGAGCGGTTG
CAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAA
GCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCAAAACTTGGGGAAAGTTC
AAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAATAAATA
TCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGC
ATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAATTGAA
AGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (46-101)

• Helix-hairpin-helix motif (HHH_3) (129-190)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	94.792	99.482	0.943
comEA/celA/cilE	Streptococcus mitis SK321	45.213	97.409	0.44
comEA	Latilactobacillus sakei subsp. sakei 23K	42.781	96.891	0.415
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.993	77.202	0.378
comEA/celA/cilE	Streptococcus pneumoniae R6	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.651	77.202	0.368
comEA	Staphylococcus aureus MW2	40	90.674	0.363
comEA	Streptococcus thermophilus LMD-9	43.478	83.42	0.363