Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   P3S72_RS04950 Genome accession   NZ_CP119772
Coordinates   1123895..1125103 (-) Length   402 a.a.
NCBI ID   WP_276196480.1    Uniprot ID   -
Organism   Pseudomonas sp. D3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1118895..1130103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3S72_RS04915 (P3S72_04915) - 1119868..1120617 (+) 750 WP_276197896.1 TIM barrel protein -
  P3S72_RS04920 (P3S72_04920) - 1120773..1121240 (+) 468 WP_276196475.1 FAD/FMN-containing dehydrogenase -
  P3S72_RS04925 (P3S72_04925) - 1121237..1121926 (+) 690 WP_276196476.1 energy-coupling factor ABC transporter permease -
  P3S72_RS04930 (P3S72_04930) - 1122003..1122218 (+) 216 WP_247396658.1 hypothetical protein -
  P3S72_RS04935 (P3S72_04935) yacG 1122215..1122415 (-) 201 WP_276196477.1 DNA gyrase inhibitor YacG -
  P3S72_RS04940 (P3S72_04940) coaE 1122412..1123035 (-) 624 WP_276196478.1 dephospho-CoA kinase -
  P3S72_RS04945 (P3S72_04945) pilD 1123032..1123898 (-) 867 WP_276196479.1 A24 family peptidase Machinery gene
  P3S72_RS04950 (P3S72_04950) pilC 1123895..1125103 (-) 1209 WP_276196480.1 type II secretion system F family protein Machinery gene
  P3S72_RS04955 (P3S72_04955) - 1125305..1125718 (+) 414 WP_276197897.1 pilin -
  P3S72_RS04960 (P3S72_04960) - 1125754..1127163 (+) 1410 WP_276196481.1 O-antigen ligase family protein -
  P3S72_RS04970 (P3S72_04970) - 1127412..1127717 (+) 306 WP_276196482.1 DUF6388 family protein -
  P3S72_RS04975 (P3S72_04975) nadC 1127761..1128609 (-) 849 WP_276196483.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43508.73 Da        Isoelectric Point: 10.1720

>NTDB_id=801107 P3S72_RS04950 WP_276196480.1 1123895..1125103(-) (pilC) [Pseudomonas sp. D3]
MNNASTTYAWEGINRQGRRVTGQAIGHDLALIKAQLRQQGIFPARVRKQSRGLPSLTPPIKPADIALFTRQLATLLKAGI
PLLQAFDVISEGFDNRHLYRLVNGLKQEIGAGNNLASALRKQPRYFNDLYCNLVAAGEQAGALETLLERVAVHLEKSERL
KARIKKAMTYPIAVIAVASLVSSLLLIHVVPQFQNLFAGVDSKLPGFTLQVIALSEFLQQAWWMLALSLGAGGFGLRQAY
RASPGFRHWLDAGLLKAPLAGTLLKKSAVARYARTLSTTFAAGVPLVQALDSVAAAAGNQLFKQAIEHMRHDVSTGMPLN
RSMASSGLFPGMAIQMTAIGEESGNLEHMLEKVASHYESDVDNLVDHLTSLMEPLIMVVLGGIVGALVIALYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=801107 P3S72_RS04950 WP_276196480.1 1123895..1125103(-) (pilC) [Pseudomonas sp. D3]
ATGAACAACGCTTCCACGACATACGCCTGGGAAGGCATCAACCGCCAGGGACGCAGGGTGACCGGGCAAGCCATCGGCCA
CGACCTCGCGCTGATCAAGGCACAACTGCGCCAGCAAGGGATTTTCCCTGCCCGCGTGCGCAAGCAATCTCGCGGCCTGC
CAAGCCTCACTCCGCCGATCAAACCGGCGGATATTGCCCTGTTCACCCGCCAGTTGGCGACGCTGCTGAAGGCCGGCATT
CCGTTACTGCAAGCCTTCGACGTCATCAGCGAAGGCTTCGACAACCGCCACCTGTACAGGCTGGTAAATGGCTTGAAACA
GGAGATCGGCGCCGGCAATAACCTGGCGTCGGCATTGCGCAAACAGCCGCGTTACTTCAATGATTTGTACTGCAACCTGG
TGGCAGCAGGTGAGCAGGCCGGCGCCCTGGAAACCCTGTTGGAGCGAGTGGCGGTTCACCTGGAAAAGAGCGAGCGGCTC
AAGGCCAGGATCAAGAAAGCCATGACCTACCCCATTGCCGTCATCGCCGTCGCCAGCCTTGTCAGCAGTCTCTTGCTGAT
CCATGTGGTGCCGCAGTTCCAGAACCTGTTCGCCGGCGTCGACAGCAAGCTGCCCGGCTTTACCCTGCAAGTGATTGCCT
TGTCCGAGTTCCTGCAACAAGCGTGGTGGATGCTGGCGCTCAGCCTGGGCGCGGGAGGTTTCGGGTTGCGCCAGGCCTAT
CGCGCATCGCCTGGTTTTCGCCATTGGCTGGATGCCGGTTTGTTGAAAGCGCCCCTGGCAGGCACACTGCTGAAAAAGTC
GGCAGTCGCCCGTTACGCCCGCACGCTTTCGACTACTTTCGCGGCCGGAGTGCCGTTGGTACAGGCCCTGGATTCAGTGG
CCGCCGCTGCCGGCAACCAGCTATTCAAACAGGCTATCGAGCATATGCGTCACGATGTATCCACAGGCATGCCGTTGAAC
CGATCCATGGCCTCCAGCGGCCTGTTTCCCGGCATGGCGATCCAGATGACGGCGATTGGTGAAGAATCCGGCAACCTGGA
GCACATGCTGGAAAAGGTCGCGAGCCACTACGAGTCTGACGTCGACAACCTGGTCGACCACCTCACCAGCCTGATGGAAC
CGCTGATCATGGTGGTGCTCGGAGGGATTGTCGGCGCCCTGGTAATCGCCCTGTACTTGCCGGTCTTCCAACTCGGTACG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.95

98.756

0.592
  pilC Acinetobacter baylyi ADP1

50.505

98.507

0.498
  pilC Legionella pneumophila strain ERS1305867

48.737

98.507

0.48
  pilC Acinetobacter baumannii D1279779

48.485

98.507

0.478
  pilC Vibrio cholerae strain A1552

39.541

97.512

0.386
  pilC Thermus thermophilus HB27

38.155

99.751

0.381
  pilG Neisseria gonorrhoeae MS11

40.327

91.294

0.368
  pilC Vibrio campbellii strain DS40M4

37.5

97.512

0.366
  pilG Neisseria meningitidis 44/76-A

39.51

91.294

0.361