Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P2C01_RS12570 Genome accession   NZ_CP119521
Coordinates   2707907..2709595 (+) Length   562 a.a.
NCBI ID   WP_275722945.1    Uniprot ID   -
Organism   Vibrio furnissii strain C1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2702907..2714595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P2C01_RS12540 dsbC 2703243..2704007 (-) 765 WP_158107763.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  P2C01_RS12545 xerD 2704024..2704932 (-) 909 WP_004729073.1 site-specific tyrosine recombinase XerD -
  P2C01_RS12550 fldB 2705074..2705595 (+) 522 WP_004729075.1 flavodoxin FldB -
  P2C01_RS12555 ampD 2705617..2706168 (-) 552 WP_275722940.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  P2C01_RS12560 nadC 2706317..2707204 (+) 888 WP_154180385.1 carboxylating nicotinate-nucleotide diphosphorylase -
  P2C01_RS12565 - 2707451..2707900 (+) 450 WP_055466517.1 pilin -
  P2C01_RS12570 pilB 2707907..2709595 (+) 1689 WP_275722945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P2C01_RS12575 pilC 2709674..2710900 (+) 1227 WP_208550263.1 type II secretion system F family protein Machinery gene
  P2C01_RS12580 pilD 2710952..2711821 (+) 870 WP_014204458.1 prepilin peptidase Machinery gene
  P2C01_RS12585 coaE 2711824..2712432 (+) 609 WP_275722948.1 dephospho-CoA kinase -
  P2C01_RS12590 zapD 2712465..2713205 (+) 741 WP_004729090.1 cell division protein ZapD -
  P2C01_RS12595 yacG 2713263..2713460 (+) 198 WP_004729092.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61932.98 Da        Isoelectric Point: 6.0779

>NTDB_id=799459 P2C01_RS12570 WP_275722945.1 2707907..2709595(+) (pilB) [Vibrio furnissii strain C1]
MLTNLIAILRQAELISPTQEQAVIDHIHASGATVPEALIDLAIFHHHDLTEQLSQIFGLPATDLTRFDYAPLCQQLGLRE
LITRYQALPLHSNGQILTLAVADPTHLQVEDEFRFATGLQVELVLADHKALHAAIHRLYGRSLATPGTQGKEISQDELAD
LVQISDDEKTTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHNRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVAITPPAALQQQFGLLAED
AVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=799459 P2C01_RS12570 WP_275722945.1 2707907..2709595(+) (pilB) [Vibrio furnissii strain C1]
ATGCTGACCAACCTTATCGCCATTCTGCGTCAGGCTGAGTTGATCAGCCCAACGCAGGAACAGGCCGTGATCGATCATAT
TCACGCTTCCGGTGCCACGGTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATTTAACCGAGCAACTAA
GTCAGATCTTTGGCCTACCCGCAACCGATCTCACTCGGTTTGATTACGCGCCATTGTGCCAGCAGTTGGGACTTCGAGAA
CTCATCACCCGCTATCAAGCCCTGCCGCTACACAGCAATGGCCAAATCCTGACGTTGGCGGTTGCTGACCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCACAAAGCGCTGCACGCCG
CCATTCACCGCTTGTATGGGCGCAGCCTCGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTGGTACAGATCTCTGACGACGAAAAAACCACCATTGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCGTCCGACATTCACTTTGAGCCGTATGAGTCCCACAACCGTATTCGTC
TGCGCTGTGACGGCATTCTGGTCGAAACCCAACAACCAGCCAGTCACCTCAGCCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAACTGGATATTGCCGAGCGCCGCCTCCCGCAAGATGGACGCATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTATGGGGTGAAAAGATTGTTCTGCGCTTACTCGATAGCAGCGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAACAGCAGAAACAGCTCTACCTCGCAGCCCTCACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACCGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGTATCAATCAGGTGCAAGTTCAACCCAAAATCGGCTTTG
GCTTTGCACAAGCGCTGCGCTCGTTTCTGCGTCAAGATCCCGATGTGGTGATGGTGGGCGAAATTCGCGATCTAGAAACC
GCAGAAATCGCCATTAAAGCCGCGCAAACCGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCGT
GATTCGTTTAGCCAACATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTCTGTGCCCGCATTGCAAGGTCGCCATCACGCCCCCAGCTGCGCTGCAGCAGCAATTTGGCTTGCTCGCCGAAGAT
GCTGTATACGAAGCACATCCATCGGGTTGCACGGAATGCACCAATGGGTATAGCGGCCGCGTGGGCATTTATGAAGTGAT
GGCGTTTCATCCCGCCTTGGCGGACGCGGTGATGCAGCGCGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GCATGCAACCCCTGCTGCAATCGGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTACAGCGAGTGCTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.028

100

0.82
  pilB Vibrio campbellii strain DS40M4

71.53

100

0.715
  pilB Vibrio parahaemolyticus RIMD 2210633

71.53

100

0.715
  pilB Legionella pneumophila strain ERS1305867

52.326

91.815

0.48
  pilB Acinetobacter baumannii D1279779

50.867

92.349

0.47
  pilB Acinetobacter baylyi ADP1

49.903

91.993

0.459
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB Haemophilus influenzae 86-028NP

39.848

93.772

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368
  pilF Thermus thermophilus HB27

36.655

100

0.367