Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PYU99_RS02495 Genome accession   NZ_CP118993
Coordinates   529353..530462 (-) Length   369 a.a.
NCBI ID   WP_270823627.1    Uniprot ID   -
Organism   Aeromonas media strain K521     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 524353..535462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYU99_RS02470 (PYU99_02470) - 524478..524705 (+) 228 WP_005332257.1 hypothetical protein -
  PYU99_RS02475 (PYU99_02475) brnQ 524794..526056 (-) 1263 WP_270823687.1 branched-chain amino acid transport system II carrier protein -
  PYU99_RS02480 (PYU99_02480) - 526293..527003 (-) 711 WP_139743691.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PYU99_RS02485 (PYU99_02485) srmB 527132..528355 (+) 1224 WP_270823625.1 ATP-dependent RNA helicase SrmB -
  PYU99_RS02490 (PYU99_02490) yaaA 528547..529320 (-) 774 WP_270823626.1 peroxide stress protein YaaA -
  PYU99_RS02495 (PYU99_02495) pilU 529353..530462 (-) 1110 WP_270823627.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PYU99_RS02500 (PYU99_02500) pilT 530489..531523 (-) 1035 WP_041206426.1 type IVa pilus ATPase TapT Machinery gene
  PYU99_RS02505 (PYU99_02505) - 531563..532264 (+) 702 WP_042649789.1 YggS family pyridoxal phosphate-dependent enzyme -
  PYU99_RS02510 (PYU99_02510) proC 532424..533251 (+) 828 WP_270823628.1 pyrroline-5-carboxylate reductase -
  PYU99_RS02515 (PYU99_02515) - 533275..533826 (+) 552 WP_025325667.1 YggT family protein -
  PYU99_RS02520 (PYU99_02520) yggU 533826..534134 (+) 309 WP_043128939.1 DUF167 family protein YggU -
  PYU99_RS02525 (PYU99_02525) - 534139..534558 (+) 420 WP_041206420.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41058.18 Da        Isoelectric Point: 6.1858

>NTDB_id=797192 PYU99_RS02495 WP_270823627.1 529353..530462(-) (pilU) [Aeromonas media strain K521]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVPLGDKPLDRQTALTLVRDSLDETHFERYLHSREANYAIHREGLGR
FRVSAFWQQDMPGMVLRRIETRIPTFEELVLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHGDGHILTVED
PVEFVHQHGRCLVTQREVGIDTDSFDVALKNSMRQAPDVILIGEIRTQETMELAIQFAETGHLCLATLHANNANQALDRI
LHLVPEAKHRQFLFDLSFNLRAIVAQQLLPNVNGSRRIAAFEILLNTPLITDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=797192 PYU99_RS02495 WP_270823627.1 529353..530462(-) (pilU) [Aeromonas media strain K521]
ATGAACATGGATGTTCTACTCGCTGCACTGGTTGAACAAAAGGGATCGGATCTCTTCATCACGGTCGATGCCCCTCCCAC
CCTCAAGGTCAATGGACGCCTGGTACCACTGGGCGATAAGCCGCTGGACAGGCAGACGGCGCTGACCCTGGTCAGGGATA
GCCTGGACGAGACGCACTTCGAGCGATACCTGCATAGCCGGGAGGCAAACTACGCCATCCACCGCGAAGGACTTGGCCGA
TTCCGGGTCAGCGCCTTCTGGCAGCAGGATATGCCGGGCATGGTGCTGCGTCGTATCGAGACCCGTATTCCCACCTTCGA
GGAGTTGGTACTGCCCCCCATCCTGCAGGATGTGGCCATGGCCAAACGCGGGCTGGTGCTGTTCGTCGGTGCCACGGGGG
CTGGCAAGTCCACCACCCAGGCGGCCATGATCGGTTATCGCAACCAGCACGGCGACGGCCACATACTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCACCAGCATGGGCGCTGCCTAGTGACCCAGCGGGAGGTCGGCATAGACACCGACTCCTTCGACGT
GGCCCTCAAGAATTCCATGCGTCAGGCACCAGATGTCATCCTGATCGGTGAGATCCGTACCCAGGAAACCATGGAACTGG
CCATCCAGTTTGCCGAGACGGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGGAGGCGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTGGCGCAGCA
ACTGCTCCCCAACGTGAATGGCAGCCGCCGGATCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATTA
TCCGCAAGGGCGAGATGCACAGGCTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGTCGCGAGCAGCTGGGTGCAGGCACACTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.511

99.729

0.593
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

56.374

95.664

0.539
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.692

89.702

0.374
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36