Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IGS56_RS03625 Genome accession   NZ_AP022846
Coordinates   718750..720144 (+) Length   464 a.a.
NCBI ID   WP_114877323.1    Uniprot ID   -
Organism   Haemophilus influenzae strain TAMBA230     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 713750..725144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGS56_RS03600 (JPHIA_06880) rsmE 713769..714506 (-) 738 WP_014550478.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IGS56_RS03605 (JPHIA_06890) lnt 714556..716085 (-) 1530 WP_080481790.1 apolipoprotein N-acyltransferase -
  IGS56_RS03610 (JPHIA_06900) corC 716108..717007 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  IGS56_RS03615 (JPHIA_06910) ampD 717638..718189 (-) 552 WP_071162800.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IGS56_RS03620 (JPHIA_06920) pilA 718304..718753 (+) 450 WP_103708055.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  IGS56_RS03625 (JPHIA_06930) pilB 718750..720144 (+) 1395 WP_114877323.1 GspE/PulE family protein Machinery gene
  IGS56_RS03630 (JPHIA_06940) pilC 720141..721358 (+) 1218 WP_167808414.1 type II secretion system F family protein Machinery gene
  IGS56_RS03635 (JPHIA_06950) pilD 721355..722047 (+) 693 WP_114877327.1 prepilin peptidase Machinery gene
  IGS56_RS03640 (JPHIA_06960) rho 722101..723363 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  IGS56_RS03645 (JPHIA_06970) metJ 723598..723915 (+) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  IGS56_RS03650 (JPHIA_06980) cueR 723929..724315 (-) 387 WP_114877329.1 Cu(I)-responsive transcriptional regulator -
  IGS56_RS03655 (JPHIA_06990) - 724392..724598 (+) 207 WP_005654130.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52873.26 Da        Isoelectric Point: 5.2105

>NTDB_id=79416 IGS56_RS03625 WP_114877323.1 718750..720144(+) (pilB) [Haemophilus influenzae strain TAMBA230]
MTSYALLHTQRVTAQNGEIFTISPDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPNQMQVEEQVKFYQHQETYFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=79416 IGS56_RS03625 WP_114877323.1 718750..720144(+) (pilB) [Haemophilus influenzae strain TAMBA230]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATATTTACGATCTCGCCTGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTGCTCTTACGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGAACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCTATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCTAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGTTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACACGCACTTAGCCAACCCCAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCGATGATTGCACTACGTGC
CGCTCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

98.491

100

0.985
  pilB Haemophilus influenzae Rd KW20

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

57.734

98.922

0.571
  pilB Vibrio cholerae strain A1552

40.038

100

0.455
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.418
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

39.085

100

0.405
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Thermus thermophilus HB27

36.008

100

0.377

Multiple sequence alignment