Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PVT68_RS06280 Genome accession   NZ_CP118605
Coordinates   1473301..1474515 (+) Length   404 a.a.
NCBI ID   WP_280321827.1    Uniprot ID   -
Organism   Microbulbifer bruguierae strain H12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1468301..1479515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT68_RS06265 (PVT68_06265) - 1469109..1470341 (-) 1233 WP_280321824.1 serine hydrolase domain-containing protein -
  PVT68_RS06270 (PVT68_06270) - 1470386..1470778 (-) 393 WP_407666144.1 pilin -
  PVT68_RS18405 - 1470793..1470904 (-) 112 Protein_1232 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PVT68_RS06275 (PVT68_06275) pilB 1471411..1473123 (+) 1713 WP_280321826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PVT68_RS06280 (PVT68_06280) pilC 1473301..1474515 (+) 1215 WP_280321827.1 type II secretion system F family protein Machinery gene
  PVT68_RS06285 (PVT68_06285) - 1474527..1475411 (+) 885 WP_280321828.1 A24 family peptidase -
  PVT68_RS06290 (PVT68_06290) coaE 1475461..1476072 (+) 612 WP_280321829.1 dephospho-CoA kinase -
  PVT68_RS06295 (PVT68_06295) yacG 1476087..1476293 (+) 207 WP_280321830.1 DNA gyrase inhibitor YacG -
  PVT68_RS06300 (PVT68_06300) - 1476374..1476871 (-) 498 WP_280321831.1 protein disulfide oxidoreductase -
  PVT68_RS06305 (PVT68_06305) tsaA 1476868..1477590 (-) 723 WP_280321832.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  PVT68_RS06310 (PVT68_06310) - 1477644..1479101 (-) 1458 WP_280321834.1 NAD-dependent succinate-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43321.02 Da        Isoelectric Point: 9.2694

>NTDB_id=793908 PVT68_RS06280 WP_280321827.1 1473301..1474515(+) (pilC) [Microbulbifer bruguierae strain H12]
MAKAAAAVAYTYKGVDAKGNKVAGEINGTNPALVKAQLRRQGIIANRVQKKPKPLFSASKKVSPSDIALFTRQLATMMKA
GVPLVQSFEIVADGSDNPGVKELISKVKDEVASGTAFADALRKHPLYFDDLFCNLVSSGEQSGALETMLDRIATYKEKTE
SLKKKIKKAMTYPVMVIVVAIVVTAILLVKVVPQFASTFAGFGAELPAFTLFVMGISEWMQEYWLISFICIVVGIGGTIE
IKKRNKNVANFFDKLMLKIPVLGLITYNSIAARFSRTLATTFAAGVPLIDALKSVAGATGNVVYEEATLKIRDSVATGIP
LNAAMGQSGLYPNMLVQMAAIGEESGALDEMLGKAADFYEEAVDNLVDNLTTMLEPMIMSILGVLVGGLLVAMYLPIFNL
GNVI

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=793908 PVT68_RS06280 WP_280321827.1 1473301..1474515(+) (pilC) [Microbulbifer bruguierae strain H12]
ATGGCCAAAGCTGCCGCCGCAGTTGCTTACACCTATAAGGGCGTGGACGCCAAAGGCAACAAAGTTGCTGGCGAGATCAA
CGGCACCAATCCGGCCCTGGTTAAGGCCCAACTACGCCGCCAGGGAATTATTGCAAACCGGGTACAGAAAAAGCCCAAAC
CGCTATTTTCTGCCAGCAAAAAAGTCTCACCTTCCGATATTGCCCTGTTTACCCGACAACTCGCCACCATGATGAAAGCC
GGTGTCCCGCTGGTACAGAGCTTTGAAATTGTCGCTGATGGCTCCGATAACCCCGGGGTAAAAGAGCTGATTTCCAAAGT
CAAAGACGAAGTTGCCTCCGGTACAGCTTTTGCCGATGCGCTACGCAAACACCCCCTGTATTTCGACGATCTGTTCTGTA
ACCTCGTCTCATCAGGTGAACAATCCGGTGCGCTCGAGACCATGCTCGACAGAATTGCCACCTACAAAGAAAAAACGGAA
TCCCTCAAGAAAAAGATCAAGAAAGCCATGACCTACCCGGTAATGGTTATCGTAGTCGCGATTGTGGTTACCGCGATTTT
GCTGGTGAAAGTTGTGCCGCAATTTGCATCGACCTTTGCCGGCTTCGGCGCAGAGCTGCCCGCATTTACGCTTTTTGTAA
TGGGAATTTCGGAGTGGATGCAGGAATACTGGTTGATATCCTTTATTTGCATTGTTGTCGGCATTGGCGGCACGATTGAA
ATAAAAAAGCGCAATAAAAATGTTGCCAACTTTTTTGACAAGCTGATGCTCAAAATTCCGGTACTTGGCCTGATCACCTA
TAACTCCATTGCCGCACGCTTTTCACGTACCCTGGCCACAACCTTTGCCGCCGGTGTTCCGTTGATTGATGCCCTCAAAT
CCGTGGCTGGGGCCACTGGCAACGTCGTATACGAAGAGGCGACGCTGAAAATTCGCGACTCCGTTGCGACCGGCATCCCT
CTCAATGCAGCAATGGGACAGTCTGGCCTCTACCCCAACATGCTGGTGCAAATGGCAGCCATCGGCGAAGAATCCGGTGC
ACTGGATGAAATGCTGGGTAAAGCGGCGGATTTTTACGAAGAAGCCGTAGACAACCTTGTAGATAACCTGACGACCATGC
TGGAGCCAATGATTATGTCCATCTTGGGCGTGCTGGTTGGCGGACTGCTGGTTGCCATGTATCTGCCAATCTTCAACCTG
GGCAACGTGATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.443

99.505

0.611
  pilC Acinetobacter baumannii D1279779

60.396

100

0.604
  pilC Acinetobacter baylyi ADP1

59.95

98.267

0.589
  pilC Legionella pneumophila strain ERS1305867

55.276

98.515

0.545
  pilG Neisseria gonorrhoeae MS11

44.444

98.02

0.436
  pilG Neisseria meningitidis 44/76-A

44.192

98.02

0.433
  pilC Vibrio cholerae strain A1552

42.424

98.02

0.416
  pilC Vibrio campbellii strain DS40M4

40.1

98.762

0.396
  pilC Thermus thermophilus HB27

37.037

100

0.371