Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PVT68_RS06275 Genome accession   NZ_CP118605
Coordinates   1471411..1473123 (+) Length   570 a.a.
NCBI ID   WP_280321826.1    Uniprot ID   -
Organism   Microbulbifer bruguierae strain H12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1466411..1478123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT68_RS06255 (PVT68_06255) - 1467050..1467295 (-) 246 WP_280321822.1 YdcH family protein -
  PVT68_RS06260 (PVT68_06260) - 1467560..1468948 (-) 1389 WP_280321823.1 glutamine synthetase family protein -
  PVT68_RS06265 (PVT68_06265) - 1469109..1470341 (-) 1233 WP_280321824.1 serine hydrolase domain-containing protein -
  PVT68_RS06270 (PVT68_06270) - 1470386..1470778 (-) 393 WP_407666144.1 pilin -
  PVT68_RS18405 - 1470793..1470904 (-) 112 Protein_1232 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PVT68_RS06275 (PVT68_06275) pilB 1471411..1473123 (+) 1713 WP_280321826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PVT68_RS06280 (PVT68_06280) pilC 1473301..1474515 (+) 1215 WP_280321827.1 type II secretion system F family protein Machinery gene
  PVT68_RS06285 (PVT68_06285) - 1474527..1475411 (+) 885 WP_280321828.1 A24 family peptidase -
  PVT68_RS06290 (PVT68_06290) coaE 1475461..1476072 (+) 612 WP_280321829.1 dephospho-CoA kinase -
  PVT68_RS06295 (PVT68_06295) yacG 1476087..1476293 (+) 207 WP_280321830.1 DNA gyrase inhibitor YacG -
  PVT68_RS06300 (PVT68_06300) - 1476374..1476871 (-) 498 WP_280321831.1 protein disulfide oxidoreductase -
  PVT68_RS06305 (PVT68_06305) tsaA 1476868..1477590 (-) 723 WP_280321832.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62379.02 Da        Isoelectric Point: 5.3441

>NTDB_id=793907 PVT68_RS06275 WP_280321826.1 1471411..1473123(+) (pilB) [Microbulbifer bruguierae strain H12]
MSSTPLSGLAKRLVSDNAIDEATAQSAIKAAKREGQTFAQHAVEAKLIKSRELANIASQAFGSPLFDLSSYNFDLLPHGI
VDEKLISKHFALPLFKRGNRLFVAVADPTNLAGLDEINFNTGLSTDAVLVEADKLAKAIESYLSNNGDIGKGLEGLDDED
LDSLSIESGDTNREEDNEPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKTYRVRLRTDGVLHEVARPPIQLATRISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEEEQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGINQVNVSNKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCGECKKPVQLPDEVLKDEGF
DTVTIPKNEWQIYQPVGCEHCSNGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQARKEGFNNLRTSALRKVTMGITS
LEEANRVTND

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=793907 PVT68_RS06275 WP_280321826.1 1471411..1473123(+) (pilB) [Microbulbifer bruguierae strain H12]
ATGAGCAGCACTCCCTTAAGTGGTCTGGCCAAGCGGCTGGTTTCTGACAATGCTATCGATGAGGCCACGGCTCAATCTGC
CATCAAGGCAGCCAAACGCGAAGGGCAAACCTTTGCCCAGCACGCGGTAGAAGCCAAGCTGATTAAAAGTCGAGAGCTGG
CCAATATCGCTTCGCAAGCGTTTGGCAGCCCATTGTTCGACCTTTCCAGCTACAACTTTGATTTACTGCCCCACGGTATT
GTTGACGAGAAGTTGATCAGCAAGCACTTTGCGCTGCCTTTGTTCAAGCGCGGTAACCGACTATTTGTCGCCGTCGCCGA
TCCCACCAATCTGGCAGGCCTCGATGAAATCAACTTCAACACCGGTCTCAGTACCGACGCGGTATTGGTTGAAGCCGACA
AGCTCGCCAAAGCCATTGAGAGCTACCTGTCTAACAACGGGGATATAGGTAAAGGCCTGGAGGGACTGGATGACGAAGAC
CTTGACAGCCTCAGCATAGAAAGCGGCGATACAAATCGTGAAGAAGACAACGAGCCCGGTGGCGACGAGGCTCCGGTTGT
ACGTTTTGTAAACAAAGTACTGCTGGATGCCATCCGTACAGGTGCCTCAGATATTCACTTTGAACCCTACGAGAAAACCT
ATCGAGTACGTTTACGCACTGATGGCGTTTTGCATGAAGTTGCCCGCCCCCCTATCCAACTGGCTACCCGAATTTCCGCC
CGCCTGAAAGTAATGTCGAAGATGGACATCTCTGAACGGCGGGTTCCCCAGGATGGACGGATCAAAATGAAGCTGTCCAA
AACCAAGGCGATTGACTTCCGGGTAAACAGCCTGCCCACCCTCTGGGGCGAGAAAATTGTTCTGCGGATTCTGGACCCCT
CCTCCGCGAAACTCGGGATTGACGCTCTGGGGTACGAGGAAGAGCAGAAGAAAATCTATATGGATGCCCTGGCTCAACCT
CAGGGCATGATCCTGGTGACAGGCCCCACTGGCTCCGGTAAAACCGTATCCCTTTATACGGGTCTGAACATTCTGAATAC
CGCTGAGCGCAATATTTCTACCGCCGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAATCAGGTTAACGTCTCCAACA
AGGTGGGACTCAATTTTGCGGAGGCACTGCGCTCCTTCCTCCGACAGGACCCTGATATTGTGATGGTGGGTGAGATTCGG
GATCTGGAAACCGCAGAAATTGCGATCAAGGCCGCCCAGACCGGTCACTTGGTGCTATCCACCCTGCATACCAACTCTGC
ACCGGAAACCCTGACCCGCCTGATGAACATGGGGGTACCAACCTTCAATATCGCCACCTCGGTAAGCGTGATTATCGCTC
AGCGGCTCGCGCGACGCCTTTGCGGGGAGTGCAAAAAGCCGGTCCAGCTGCCAGACGAAGTGCTGAAAGACGAGGGTTTT
GATACGGTCACCATCCCAAAGAATGAATGGCAAATTTACCAGCCTGTCGGTTGCGAGCACTGCTCCAATGGCTATAAGGG
ACGCGTAGGTGTGTATGAAGTAGTTCGCATAACTGACGGAATTTCACGCATTATAATGGAGGGTGGTAATTCCATTCAGA
TTGCCGATCAGGCAAGGAAGGAGGGGTTCAATAACCTCCGCACCTCGGCACTGCGCAAGGTGACAATGGGTATCACCAGT
CTCGAAGAGGCCAACCGGGTTACCAACGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.825

100

0.598
  pilB Acinetobacter baylyi ADP1

58.844

100

0.589
  pilB Legionella pneumophila strain ERS1305867

54.497

99.474

0.542
  pilB Vibrio cholerae strain A1552

51.413

99.298

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

53.309

95.439

0.509
  pilB Vibrio campbellii strain DS40M4

52.198

95.789

0.5
  pilF Neisseria gonorrhoeae MS11

50.947

92.632

0.472
  pilB/pilB1 Synechocystis sp. PCC 6803

38.374

100

0.414
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.104

98.07

0.374