Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PWW22_RS12100 Genome accession   NZ_CP118578
Coordinates   2688984..2690207 (+) Length   407 a.a.
NCBI ID   WP_005446256.1    Uniprot ID   K5TX94
Organism   Vibrio harveyi strain fish2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2683984..2695207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW22_RS12080 ampD 2685041..2685592 (-) 552 WP_050936250.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWW22_RS12085 nadC 2685685..2686572 (+) 888 WP_045393857.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWW22_RS12090 pilA 2686837..2687265 (+) 429 WP_009698754.1 pilin Machinery gene
  PWW22_RS12095 pilB 2687265..2688950 (+) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWW22_RS12100 pilC 2688984..2690207 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWW22_RS12105 pilD 2690280..2691149 (+) 870 WP_045488712.1 A24 family peptidase Machinery gene
  PWW22_RS12110 coaE 2691150..2691764 (+) 615 WP_017817675.1 dephospho-CoA kinase -
  PWW22_RS12115 zapD 2691792..2692532 (+) 741 WP_274880413.1 cell division protein ZapD -
  PWW22_RS12120 yacG 2692589..2692789 (+) 201 WP_026000212.1 DNA gyrase inhibitor YacG -
  PWW22_RS12125 rplS 2693156..2693509 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  PWW22_RS12130 trmD 2693551..2694294 (-) 744 WP_005446247.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PWW22_RS12135 rimM 2694322..2694870 (-) 549 WP_005446245.1 ribosome maturation factor RimM -
  PWW22_RS12140 rpsP 2694899..2695147 (-) 249 WP_004410028.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44945.20 Da        Isoelectric Point: 10.3829

>NTDB_id=793519 PWW22_RS12100 WP_005446256.1 2688984..2690207(+) (pilC) [Vibrio harveyi strain fish2]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEVEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITVFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTKVIPEFEKMFTGFGAELPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLTARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=793519 PWW22_RS12100 WP_005446256.1 2688984..2690207(+) (pilC) [Vibrio harveyi strain fish2]
ATGAAAACCATCGCTCCACAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGAAAAAAAACCTCTGGTAATGT
GCTCGCAATGACAGAAGTCGAGGTAAGAGAGCGCCTTGATGCTCAGCACATCAAGATAAAAAAACTCAAAAAAGGCAGTA
TTTCTTTTATCACCAAGATAAGTCATCGAGTAAAAGGCAAAGACATTACGGTGTTTACTCGTCAAATAGCCACCATGTTG
ATGACTGGCGTCCCTATTGTTCAAGCACTCAAGTTAGTTTCAGAGAACCATAAAAAAGCTGAAATGAAATCGATCCTAAT
GAGTGTGACGCGAGCGGTAGAAGCAGGGACACCGATGTCGAAAGCCATGCGTACCGCTAGCACGCACTTTGATCCACTTT
ACACCGACTTAATAGCAACAGGTGAGCAGTCCGGTAACTTATCCCAAGTCTTTGAGCGTCTGGCAACCTACCGAGAAAAA
AGCGAGCAGTTGCGTGCAAAGGTTATTAAAGCGCTCATTTACCCAGCGATGGTGGTATTAGTAGCACTTGGGGTTTCTTA
TTTAATGCTGACAAAGGTTATTCCTGAGTTTGAAAAAATGTTTACAGGTTTTGGTGCCGAATTACCCGCCTTCACCCAGA
TGGTACTCAACCTTTCTGCTTGGACCCAAAATTGGGGTCCCTTCATTGGGCTGAGCTTTGTGAGCTTACTCATTTCTGGC
AAAATCATGTCACAACGCTCCGACTCATTTCGTCTTTTGACTGCACGGTCGAGTTTAAGAGTACCTATTTTAGGACCAGT
TTTATCCAAAGCTGCCATTGCGAAGTTTAGCCGTACTTTGGCGACCAGTTTTAGCGCAGGTATTCCTATCCTAACCGCCT
TGAAAACTACCTCAAAAACCTCTGGTAACATGCACTATCAATTGGCAATCGAAGAGGTATATCGAGATACTGCCGCTGGT
ATGCCAATGTACATTGCCATGCGTAACTGTAACGTCTTCCCAGAATTGGTATTGCAAATGGTCATGATTGGTGAAGAGTC
AGGTCGCTTAGATGACATGCTCAACAAGATAGCCGCTATTTATGAGTTCGAAGTCGATAACACCGTCGACAACCTAAGTA
AGATTTTGGAACCGTTGATCATTGTTTTTCTGGGTGTCGTTGTCGGTGGTTTGGTGACAGCAATGTACTTACCAATCTTT
AACTTGATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K5TX94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

98.28

100

0.983
  pilC Vibrio cholerae strain A1552

74

98.28

0.727
  pilC Acinetobacter baylyi ADP1

42.462

97.789

0.415
  pilC Pseudomonas stutzeri DSM 10701

41.414

97.297

0.403
  pilC Acinetobacter baumannii D1279779

40.447

99.017

0.4
  pilG Neisseria gonorrhoeae MS11

39.558

100

0.396
  pilC Legionella pneumophila strain ERS1305867

39.163

99.754

0.391
  pilG Neisseria meningitidis 44/76-A

38.725

100

0.388