Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PWW22_RS12090 Genome accession   NZ_CP118578
Coordinates   2686837..2687265 (+) Length   142 a.a.
NCBI ID   WP_009698754.1    Uniprot ID   A0A8B3DN92
Organism   Vibrio harveyi strain fish2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2681837..2692265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW22_RS12075 pdhR 2683869..2684636 (-) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PWW22_RS12080 ampD 2685041..2685592 (-) 552 WP_050936250.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWW22_RS12085 nadC 2685685..2686572 (+) 888 WP_045393857.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWW22_RS12090 pilA 2686837..2687265 (+) 429 WP_009698754.1 pilin Machinery gene
  PWW22_RS12095 pilB 2687265..2688950 (+) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWW22_RS12100 pilC 2688984..2690207 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWW22_RS12105 pilD 2690280..2691149 (+) 870 WP_045488712.1 A24 family peptidase Machinery gene
  PWW22_RS12110 coaE 2691150..2691764 (+) 615 WP_017817675.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14524.57 Da        Isoelectric Point: 5.7554

>NTDB_id=793517 PWW22_RS12090 WP_009698754.1 2686837..2687265(+) (pilA) [Vibrio harveyi strain fish2]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSEAAAGLATLRSLTTNIDTFIADTGTFPADADAPTLGAA
VDMNKLGTISFADSGASGATATFTFDGTASALASTDTVVLAKDATTGLWTCSHSTGVTLKGC

Nucleotide


Download         Length: 429 bp        

>NTDB_id=793517 PWW22_RS12090 WP_009698754.1 2686837..2687265(+) (pilA) [Vibrio harveyi strain fish2]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTGATGATAGTGGTAGCGGTTATTGGTGTTTT
AGCAGCGATTGCTATTCCACAGTATCAGAATTATGTAAAAAAATCAGAGGCTGCAGCTGGGCTTGCAACTCTTCGTTCAC
TAACTACAAATATTGATACCTTCATTGCTGATACTGGCACCTTTCCTGCTGATGCCGACGCACCCACATTAGGTGCTGCT
GTTGATATGAACAAACTTGGAACAATTTCTTTTGCAGATAGTGGTGCATCGGGAGCGACAGCAACGTTTACTTTTGATGG
TACAGCTAGCGCACTAGCTTCTACTGATACCGTTGTCCTTGCGAAGGATGCAACTACCGGACTTTGGACCTGCTCTCATA
GTACTGGCGTAACTCTAAAAGGCTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B3DN92

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.299

100

0.556
  pilA Vibrio cholerae strain A1552

51.299

100

0.556
  pilA Vibrio cholerae C6706

51.299

100

0.556
  pilA Vibrio parahaemolyticus RIMD 2210633

48.951

100

0.493
  pilA Pseudomonas aeruginosa PAK

45.27

100

0.472
  pilA Acinetobacter baumannii strain A118

35.294

100

0.38