Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PWW27_RS10710 Genome accession   NZ_CP118576
Coordinates   2322833..2324056 (+) Length   407 a.a.
NCBI ID   WP_005446256.1    Uniprot ID   K5TX94
Organism   Vibrio harveyi strain fish1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2317833..2329056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW27_RS10690 ampD 2318850..2319401 (-) 552 WP_017189135.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWW27_RS10695 nadC 2319494..2320381 (+) 888 WP_274885710.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWW27_RS10700 pilA 2320646..2321110 (+) 465 WP_005446258.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  PWW27_RS10705 pilB 2321114..2322799 (+) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWW27_RS10710 pilC 2322833..2324056 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWW27_RS10715 pilD 2324129..2324998 (+) 870 WP_110415384.1 A24 family peptidase Machinery gene
  PWW27_RS10720 coaE 2324999..2325613 (+) 615 WP_110415385.1 dephospho-CoA kinase -
  PWW27_RS10725 zapD 2325641..2326381 (+) 741 WP_005446251.1 cell division protein ZapD -
  PWW27_RS10730 yacG 2326438..2326638 (+) 201 WP_026000212.1 DNA gyrase inhibitor YacG -
  PWW27_RS10735 rplS 2327005..2327358 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  PWW27_RS10740 trmD 2327400..2328143 (-) 744 WP_005446247.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PWW27_RS10745 rimM 2328171..2328719 (-) 549 WP_005446245.1 ribosome maturation factor RimM -
  PWW27_RS10750 rpsP 2328748..2328996 (-) 249 WP_004410028.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44945.20 Da        Isoelectric Point: 10.3829

>NTDB_id=793472 PWW27_RS10710 WP_005446256.1 2322833..2324056(+) (pilC) [Vibrio harveyi strain fish1]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEVEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITVFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTKVIPEFEKMFTGFGAELPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLTARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=793472 PWW27_RS10710 WP_005446256.1 2322833..2324056(+) (pilC) [Vibrio harveyi strain fish1]
ATGAAAACCATCGCTCCACAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGAAAAAAAACCTCTGGTAACGT
GCTCGCAATGACAGAAGTCGAGGTAAGAGAGCGCCTTGATGCTCAGCACATCAAGATAAAAAAACTCAAAAAAGGCAGTA
TTTCTTTTATCACCAAGATAAGTCATCGAGTAAAAGGCAAAGACATTACGGTGTTTACTCGTCAAATCGCCACCATGTTG
ATGACTGGCGTCCCTATTGTTCAGGCACTCAAGTTAGTTTCAGAGAACCATAAAAAAGCTGAAATGAAGTCGATCCTAAT
GAGTGTGACGCGAGCGGTAGAAGCAGGGACACCGATGTCGAAAGCCATGCGTACCGCTAGCACGCACTTTGATCCACTTT
ACACCGACTTAATAGCAACAGGTGAGCAGTCCGGTAACTTATCCCAAGTCTTTGAGCGTCTGGCAACCTACCGAGAAAAA
AGCGAGCAGTTGCGTGCAAAGGTTATTAAAGCGCTCATTTACCCGGCGATGGTGGTATTAGTAGCACTTGGGGTTTCTTA
TTTAATGCTGACAAAGGTTATTCCTGAGTTTGAAAAAATGTTTACAGGTTTTGGTGCCGAATTACCCGCCTTCACCCAGA
TGGTACTCAACCTTTCTGCTTGGACCCAAAATTGGGGTCCCTTCATTGGGCTGAGCTTTGTGAGCTTACTCATTTCTGGC
AAAATCATGTCACAACGCTCCGACTCATTTCGTCTTTTGACTGCACGGTCGAGTTTAAGAGTACCTATTTTAGGACCAGT
TTTATCCAAAGCTGCCATTGCGAAGTTTAGCCGTACTTTGGCGACCAGTTTTAGCGCAGGTATTCCTATCCTAACCGCCT
TGAAAACTACCTCAAAAACCTCTGGTAACATGCATTATCAATTGGCAATCGAAGAGGTATATCGAGATACTGCCGCTGGT
ATGCCAATGTACATTGCCATGCGTAACTGTAACGTCTTCCCAGAATTGGTATTGCAAATGGTCATGATTGGTGAAGAGTC
AGGTCGCTTAGATGACATGCTCAACAAGATAGCCGCTATTTATGAGTTCGAAGTCGATAACACCGTCGACAACCTAAGTA
AGATTTTGGAACCGTTGATCATTGTTTTTCTGGGTGTCGTTGTCGGTGGTTTGGTGACAGCAATGTACTTACCAATCTTT
AACTTGATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K5TX94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

98.28

100

0.983
  pilC Vibrio cholerae strain A1552

74

98.28

0.727
  pilC Acinetobacter baylyi ADP1

42.462

97.789

0.415
  pilC Pseudomonas stutzeri DSM 10701

41.414

97.297

0.403
  pilC Acinetobacter baumannii D1279779

40.447

99.017

0.4
  pilG Neisseria gonorrhoeae MS11

39.558

100

0.396
  pilC Legionella pneumophila strain ERS1305867

39.163

99.754

0.391
  pilG Neisseria meningitidis 44/76-A

38.725

100

0.388