Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PWW27_RS10700 Genome accession   NZ_CP118576
Coordinates   2320646..2321110 (+) Length   154 a.a.
NCBI ID   WP_005446258.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2315646..2326110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW27_RS10685 pdhR 2317678..2318445 (-) 768 WP_110415382.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PWW27_RS10690 ampD 2318850..2319401 (-) 552 WP_017189135.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWW27_RS10695 nadC 2319494..2320381 (+) 888 WP_274885710.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWW27_RS10700 pilA 2320646..2321110 (+) 465 WP_005446258.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  PWW27_RS10705 pilB 2321114..2322799 (+) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWW27_RS10710 pilC 2322833..2324056 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWW27_RS10715 pilD 2324129..2324998 (+) 870 WP_110415384.1 A24 family peptidase Machinery gene
  PWW27_RS10720 coaE 2324999..2325613 (+) 615 WP_110415385.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16024.27 Da        Isoelectric Point: 5.8347

>NTDB_id=793470 PWW27_RS10700 WP_005446258.1 2320646..2321110(+) (pilA) [Vibrio harveyi strain fish1]
MKTNKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKAAAAMKLAVELCAHENASDAATFKTNCLSGS
NGVPSKIADLNDISIEAVAGSSGVNVIAKAIAVKGPIAANEQYTMASNYTASGIEWTGSCQDSGGNAQTDYCPE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=793470 PWW27_RS10700 WP_005446258.1 2320646..2321110(+) (pilA) [Vibrio harveyi strain fish1]
ATGAAAACGAATAAACAGAAGAAGCAGCAGGGTTTTACTCTAATTGAATTGATGATTGTGGTTGCAATTATTGGTATTTT
GGCTGCATTTGCAGTACCTGCATATCAAAACTATACAAAAAAAGCGACATTAGCGGAGTTCCCAAAAGCAGCAGCAGCTA
TGAAGTTAGCAGTTGAATTGTGCGCGCACGAAAATGCTTCAGATGCAGCGACCTTTAAAACAAACTGCCTTTCTGGTAGC
AATGGAGTGCCTTCAAAGATTGCTGATTTAAACGATATATCTATTGAAGCTGTCGCTGGTTCCTCTGGCGTAAATGTCAT
TGCAAAAGCAATTGCAGTGAAAGGACCTATCGCAGCTAATGAACAATACACAATGGCATCAAACTACACTGCAAGTGGTA
TTGAATGGACTGGCTCTTGTCAGGATTCTGGGGGGAACGCTCAGACTGATTACTGTCCTGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.774

100

0.571
  pilA Haemophilus influenzae 86-028NP

41.958

92.857

0.39
  pilA2 Legionella pneumophila strain ERS1305867

39.865

96.104

0.383
  pilA2 Legionella pneumophila str. Paris

39.865

96.104

0.383