Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   Q7589_RS25175 Genome accession   NZ_CP131785
Coordinates   5373751..5374215 (-) Length   154 a.a.
NCBI ID   WP_023092872.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2014S09-209     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5368751..5379215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7589_RS25160 (Q7589_25165) nadC 5371253..5372101 (+) 849 WP_033995373.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Q7589_RS25170 (Q7589_25175) - 5372282..5373595 (-) 1314 WP_231280937.1 O-antigen ligase family protein -
  Q7589_RS25175 (Q7589_25180) pilA/pilAI 5373751..5374215 (-) 465 WP_023092872.1 pilin Machinery gene
  Q7589_RS25180 (Q7589_25185) pilB 5374446..5376146 (+) 1701 WP_023092873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q7589_RS25185 (Q7589_25190) pilC 5376150..5377367 (+) 1218 WP_033995372.1 type II secretion system F family protein Machinery gene
  Q7589_RS25190 (Q7589_25195) pilD 5377368..5378240 (+) 873 WP_023092875.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  Q7589_RS25195 (Q7589_25200) coaE 5378237..5378848 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  Q7589_RS25200 (Q7589_25205) yacG 5378845..5379045 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16090.43 Da        Isoelectric Point: 8.9912

>NTDB_id=793233 Q7589_RS25175 WP_023092872.1 5373751..5374215(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 2014S09-209]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGGSSGKGIAGAVITVKRDDKGVWTCGITGKPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=793233 Q7589_RS25175 WP_023092872.1 5373751..5374215(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 2014S09-209]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGTAAGAAGCTCGTTTCTAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCGGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGAAAGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.161

100

0.455
  pilA Pseudomonas aeruginosa PAK

41.772

100

0.429
  pilA Acinetobacter baumannii strain A118

45.07

92.208

0.416
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364
  pilA2 Legionella pneumophila strain ERS1305867

37.838

96.104

0.364