Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PWS04_RS09485 Genome accession   NZ_CP118528
Coordinates   1951626..1952849 (-) Length   407 a.a.
NCBI ID   WP_005446256.1    Uniprot ID   K5TX94
Organism   Vibrio harveyi strain 94/17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1946626..1957849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWS04_RS09450 rimM 1946764..1947312 (+) 549 WP_005446245.1 ribosome maturation factor RimM -
  PWS04_RS09455 trmD 1947340..1948083 (+) 744 WP_005446247.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PWS04_RS09460 rplS 1948125..1948478 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  PWS04_RS09465 yacG 1949044..1949244 (-) 201 WP_026000212.1 DNA gyrase inhibitor YacG -
  PWS04_RS09470 zapD 1949301..1950041 (-) 741 WP_038897986.1 cell division protein ZapD -
  PWS04_RS09475 coaE 1950069..1950683 (-) 615 WP_050922069.1 dephospho-CoA kinase -
  PWS04_RS09480 pilD 1950684..1951553 (-) 870 WP_045488712.1 A24 family peptidase Machinery gene
  PWS04_RS09485 pilC 1951626..1952849 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWS04_RS09490 pilB 1952883..1954568 (-) 1686 WP_005446257.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWS04_RS09495 - 1954568..1955035 (-) 468 WP_050933475.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PWS04_RS09500 nadC 1955300..1956187 (-) 888 WP_199449848.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWS04_RS09505 ampD 1956280..1956831 (+) 552 WP_154203416.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44945.20 Da        Isoelectric Point: 10.3829

>NTDB_id=792896 PWS04_RS09485 WP_005446256.1 1951626..1952849(-) (pilC) [Vibrio harveyi strain 94/17]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEVEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITVFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTKVIPEFEKMFTGFGAELPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLTARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=792896 PWS04_RS09485 WP_005446256.1 1951626..1952849(-) (pilC) [Vibrio harveyi strain 94/17]
ATGAAAACCATCGCTCCACAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGTAAAAAAACCTCTGGTAATGT
GCTCGCAATGACAGAAGTCGAGGTAAGAGAGCGCCTTGATGCTCAGCACATCAAGATAAAAAAACTCAAAAAAGGCAGTA
TTTCTTTTATCACCAAGATAAGTCATCGAGTAAAAGGCAAAGACATTACGGTGTTTACTCGTCAAATCGCCACCATGTTG
ATGACTGGCGTCCCTATTGTTCAGGCACTCAAGTTAGTTTCAGAGAACCATAAAAAAGCTGAAATGAAGTCGATTCTAAT
GAGTGTGACGCGAGCGGTAGAAGCAGGGACACCGATGTCGAAAGCCATGCGTACCGCTAGCACGCACTTTGATCCACTTT
ACACCGACTTAATAGCAACAGGTGAGCAGTCCGGTAACTTATCCCAAGTCTTTGAGCGTCTGGCAACCTACCGAGAAAAA
AGCGAGCAGTTGCGTGCAAAGGTTATTAAAGCGCTCATTTACCCGGCGATGGTGGTATTAGTAGCACTTGGGGTTTCTTA
TTTAATGCTGACAAAGGTTATTCCTGAGTTTGAAAAAATGTTTACAGGTTTTGGTGCCGAATTACCCGCCTTCACCCAGA
TGGTACTCAACCTTTCTGCTTGGACCCAAAATTGGGGTCCCTTCATTGGGCTGAGCTTTGTGAGCTTACTCATTTCTGGC
AAAATCATGTCACAACGCTCCGACTCATTTCGTCTTTTGACTGCACGGTCGAGTTTAAGAGTACCTATTTTAGGACCAGT
TTTATCCAAAGCTGCCATTGCGAAGTTTAGCCGTACTTTGGCGACCAGTTTTAGCGCAGGTATTCCTATCCTAACCGCCT
TGAAAACTACCTCAAAAACCTCTGGTAACATGCACTATCAATTGGCAATCGAAGAGGTATATCGAGATACTGCCGCTGGT
ATGCCAATGTACATTGCCATGCGTAACTGTAACGTCTTCCCAGAATTGGTATTGCAAATGGTCATGATTGGTGAAGAGTC
AGGTCGCTTAGATGACATGCTCAACAAGATAGCCGCTATTTATGAGTTCGAAGTCGATAACACCGTCGACAACCTAAGTA
AGATTTTGGAACCGTTGATCATTGTTTTTCTGGGTGTCGTTGTCGGTGGTTTGGTGACAGCAATGTACTTACCAATCTTT
AACTTGATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K5TX94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

98.28

100

0.983
  pilC Vibrio cholerae strain A1552

74

98.28

0.727
  pilC Acinetobacter baylyi ADP1

42.462

97.789

0.415
  pilC Pseudomonas stutzeri DSM 10701

41.414

97.297

0.403
  pilC Acinetobacter baumannii D1279779

40.447

99.017

0.4
  pilG Neisseria gonorrhoeae MS11

39.558

100

0.396
  pilC Legionella pneumophila strain ERS1305867

39.163

99.754

0.391
  pilG Neisseria meningitidis 44/76-A

38.725

100

0.388