Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   PWK66_RS00660 Genome accession   NZ_CP118482
Coordinates   101843..102781 (+) Length   312 a.a.
NCBI ID   WP_002987773.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 20160179     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 96843..107781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWK66_RS00650 (PWK66_00640) rpoC 97610..101233 (+) 3624 WP_011527438.1 DNA-directed RNA polymerase subunit beta' -
  PWK66_RS00655 (PWK66_00645) - 101385..101750 (+) 366 WP_002986560.1 DUF1033 family protein -
  PWK66_RS00660 (PWK66_00650) comYA 101843..102781 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  PWK66_RS00665 (PWK66_00655) comGB 102717..103751 (+) 1035 Protein_82 competence type IV pilus assembly protein ComGB -
  PWK66_RS00670 (PWK66_00660) comYC 103753..104079 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  PWK66_RS00675 (PWK66_00665) comGD 104054..104482 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  PWK66_RS00680 (PWK66_00670) comGE 104439..104723 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  PWK66_RS00685 (PWK66_00675) comYF 104716..105150 (+) 435 WP_002987783.1 competence type IV pilus minor pilin ComGF Machinery gene
  PWK66_RS00690 (PWK66_00680) comGG 105134..105460 (+) 327 WP_002987787.1 competence type IV pilus minor pilin ComGG -
  PWK66_RS00695 (PWK66_00685) comYH 105558..106511 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  PWK66_RS00700 (PWK66_00690) - 106570..107766 (+) 1197 WP_002987791.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35046.30 Da        Isoelectric Point: 8.5799

>NTDB_id=792698 PWK66_RS00660 WP_002987773.1 101843..102781(+) (comYA) [Streptococcus pyogenes strain 20160179]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGITKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=792698 PWK66_RS00660 WP_002987773.1 101843..102781(+) (comYA) [Streptococcus pyogenes strain 20160179]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAAGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAGTTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAAATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTATAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.45

99.679

0.603
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

40

92.949

0.372