Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   PWK66_RS00695 Genome accession   NZ_CP118482
Coordinates   105558..106511 (+) Length   317 a.a.
NCBI ID   WP_002987790.1    Uniprot ID   A0A5S4TCX5
Organism   Streptococcus pyogenes strain 20160179     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100558..111511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWK66_RS00655 (PWK66_00645) - 101385..101750 (+) 366 WP_002986560.1 DUF1033 family protein -
  PWK66_RS00660 (PWK66_00650) comYA 101843..102781 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  PWK66_RS00665 (PWK66_00655) comGB 102717..103751 (+) 1035 Protein_82 competence type IV pilus assembly protein ComGB -
  PWK66_RS00670 (PWK66_00660) comYC 103753..104079 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  PWK66_RS00675 (PWK66_00665) comGD 104054..104482 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  PWK66_RS00680 (PWK66_00670) comGE 104439..104723 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  PWK66_RS00685 (PWK66_00675) comYF 104716..105150 (+) 435 WP_002987783.1 competence type IV pilus minor pilin ComGF Machinery gene
  PWK66_RS00690 (PWK66_00680) comGG 105134..105460 (+) 327 WP_002987787.1 competence type IV pilus minor pilin ComGG -
  PWK66_RS00695 (PWK66_00685) comYH 105558..106511 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  PWK66_RS00700 (PWK66_00690) - 106570..107766 (+) 1197 WP_002987791.1 acetate kinase -
  PWK66_RS00705 (PWK66_00695) - 107953..108261 (+) 309 WP_011284425.1 hypothetical protein -
  PWK66_RS00710 (PWK66_00700) proC 108344..109114 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -
  PWK66_RS00715 (PWK66_00705) pepA 109162..110229 (-) 1068 WP_002987798.1 glutamyl aminopeptidase -
  PWK66_RS00720 (PWK66_00710) - 110694..110975 (+) 282 WP_024623420.1 DUF4651 domain-containing protein -
  PWK66_RS00725 (PWK66_00715) - 110975..111292 (+) 318 WP_002987805.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35985.08 Da        Isoelectric Point: 4.7205

>NTDB_id=792701 PWK66_RS00695 WP_002987790.1 105558..106511(+) (comYH) [Streptococcus pyogenes strain 20160179]
MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEGASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQT
EQLQANHQFTPDAIGFILLYLLEQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSLKYLKKDGFAIFLAPVNLLTSPQ
SQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIVLQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=792701 PWK66_RS00695 WP_002987790.1 105558..106511(+) (comYH) [Streptococcus pyogenes strain 20160179]
ATGACTTTTGAAAAAATTGAAGAAGCTTATCAGCTGCTCTTAGAGAACTGTCAACTAATTGAAAATGATCTAAAAACACA
TATATACGATGCCATTGTTGAACAAAACTCCTTTTATTTAGGGGCTGAGGGAGCTAGTCCTCAAGTTGCTCAAAATAGTG
ATAAACTGAAAGCCTTGTGCCTGACAAAAGAAGAATGGCGTAAGGCCTACCAGTTTCTTTTTATTAAGGCAGCTCAGACT
GAGCAACTCCAAGCCAACCATCAGTTCACACCAGATGCTATTGGCTTCATTCTGCTGTATCTTTTGGAACAATTGAGTGA
TAAAGATAGCTTAGAGGTACTTGAGATTGGAAGTGGAACAGGGAACCTAGCCCAAACCCTTCTCAACAACACGAGCAAGA
GCCTTGATTATGTAGGGATTGAACTTGATGATCTCTTGATTGATCTGTCAGCCAGTATTGCTGAGATAATGGATTCTTCA
GCTCATTTTATTCAAGAAGATGCGGTAAGGCCTCAATTACTAAAAGAAAGTGACATTGTCATCAGTGACTTACCAGTTGG
TTATTATCCTAACGATGATATTGCCAAACGGTACAAGGTGGCTAGTTCAGATAAGCATACCTATGCCCATCATTTATTAA
TGGAACAGTCTTTAAAATACTTGAAAAAAGACGGTTTTGCGATTTTTCTGGCACCAGTCAATTTATTGACGAGCCCTCAG
AGTCAGTTATTGAAACAGTGGTTAAAAGATTATGCTCAGGTGGTGGCCTTGATTACGCTACCAGATTCTATTTTTGGTCA
TCCCTCAAATGCCAAGTCCATTATTGTCTTACAAAAACAAACAGACCACCCAATGGAAACCTTTGTCTATCCAATTCGGG
ATTTGAAGCTTGCAGAGAATATTCATGATTTTATGGAAAATTTCAAAAAATGGAAACTGAGTAATGTCAATTAA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(68-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TCX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

66.139

99.685

0.659
  comYH Streptococcus mutans UA140

65.823

99.685

0.656