Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PS1M3_RS14035 Genome accession   NZ_AP022624
Coordinates   3004641..3005681 (-) Length   346 a.a.
NCBI ID   WP_006793191.1    Uniprot ID   A0A0W1KUI3
Organism   Pseudoalteromonas sp. PS1M3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2999641..3010681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS1M3_RS14005 (PS1M3_27360) - 2999809..3000285 (+) 477 WP_006793185.1 hypothetical protein -
  PS1M3_RS14010 (PS1M3_27370) rsmE 3000360..3001094 (+) 735 WP_008132394.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PS1M3_RS14015 (PS1M3_27380) gshB 3001171..3002121 (+) 951 WP_008132396.1 glutathione synthase -
  PS1M3_RS14020 (PS1M3_27390) - 3002292..3002849 (+) 558 WP_008132397.1 YqgE/AlgH family protein -
  PS1M3_RS14025 (PS1M3_27400) ruvX 3002978..3003427 (+) 450 WP_006793189.1 Holliday junction resolvase RuvX -
  PS1M3_RS14030 (PS1M3_27410) pilU 3003501..3004631 (-) 1131 WP_203985029.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PS1M3_RS14035 (PS1M3_27420) pilT 3004641..3005681 (-) 1041 WP_006793191.1 type IV pilus twitching motility protein PilT Machinery gene
  PS1M3_RS14040 (PS1M3_27430) - 3005684..3006397 (+) 714 WP_008132401.1 YggS family pyridoxal phosphate-dependent enzyme -
  PS1M3_RS14045 (PS1M3_27440) proC 3006415..3007236 (+) 822 WP_008132402.1 pyrroline-5-carboxylate reductase -
  PS1M3_RS14050 (PS1M3_27450) - 3007264..3007797 (+) 534 WP_006793194.1 YggT family protein -
  PS1M3_RS14055 (PS1M3_27460) - 3007857..3008303 (+) 447 WP_008132403.1 DUF4426 domain-containing protein -
  PS1M3_RS14060 (PS1M3_27470) - 3008308..3008907 (+) 600 WP_008132404.1 XTP/dITP diphosphatase -
  PS1M3_RS14065 (PS1M3_27480) hemW 3008904..3010040 (+) 1137 WP_008132405.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38036.28 Da        Isoelectric Point: 6.1262

>NTDB_id=78975 PS1M3_RS14035 WP_006793191.1 3004641..3005681(-) (pilT) [Pseudoalteromonas sp. PS1M3]
MDITELLAFSVQHNASDLHLSSGVSPMIRVDGDVRRINIPALADKDVNSLVYDIMNDTQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFKTISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQNKHHHILTIED
PIEFVHDNKLSLVNQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSSIQTGASIGMQTMDQCL
TNLVNRGIVTNTAAQAKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=78975 PS1M3_RS14035 WP_006793191.1 3004641..3005681(-) (pilT) [Pseudoalteromonas sp. PS1M3]
ATGGATATTACCGAATTATTAGCGTTTAGTGTGCAACACAATGCATCCGATTTACATTTATCGTCGGGTGTTTCACCTAT
GATACGCGTAGATGGTGATGTTCGCCGCATTAATATACCAGCCCTTGCAGATAAAGACGTAAACAGTTTAGTTTACGATA
TTATGAACGATACTCAGCGCAAAGATTACGAACAAAACCTTGAGGTGGATTTTTCGTTTGAGGTTCCTAATCTTGCTCGC
TTTCGTGTTAATGCCTTTAATTCAAACCGCGGTCCTGCGGCAGTATTTCGTACTATACCGAGTGAAGTATTAACACTTGA
TGATTTAGGCGCGCCAGATATTTTTAAAACAATATCAGACAACCCGCGTGGGTTAGTGCTTGTTACCGGGCCAACAGGGT
CGGGTAAATCAACAACACTTGCGGCCATGGTTGATTACATAAATCAAAATAAGCATCACCATATTTTAACGATTGAAGAC
CCAATCGAATTTGTTCACGATAACAAGTTAAGCTTAGTTAACCAACGTGAAGTGCATCGTGATACACACAGTTTTTCAAA
CGCACTACGTAGTGCGCTGCGTGAAGACCCCGATGTTATATTAGTGGGTGAGTTGCGTGATTTAGAAACTATTCGCCTAG
CGATGACCGCTGCTGAAACCGGTCATTTAGTGTTTGGTACGTTGCATACAACCTCAGCGCCCAAAACCATTGACCGTATT
ATTGATGTATTCCCTGGTGAAGAAAAAGCAATGGTACGCTCTATGTTGTCTGAGTCATTGCGTGCGGTTATTTCACAAAC
ACTCCTTAAAAAAATTGGCGGTGGGCGAGTGGCTGCTCATGAAATTATGATTGGTGTACCCGCCATACGTAACTTAATTC
GTGAAGACAAAATTGCACAAATGTACTCGTCTATTCAAACGGGGGCATCAATTGGTATGCAAACAATGGATCAATGTTTA
ACAAACTTAGTTAACCGAGGCATTGTGACCAACACCGCAGCGCAAGCTAAAGCGCAAGACAAAACACAATTTGGTGGTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W1KUI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.422

0.76
  pilT Acinetobacter nosocomialis M2

76.453

99.422

0.76
  pilT Acinetobacter baumannii D1279779

76.453

99.422

0.76
  pilT Acinetobacter baumannii strain A118

76.453

99.422

0.76
  pilT Pseudomonas aeruginosa PAK

76.163

99.422

0.757
  pilT Acinetobacter baylyi ADP1

75.581

99.422

0.751
  pilT Legionella pneumophila strain ERS1305867

72.384

99.422

0.72
  pilT Legionella pneumophila strain Lp02

72.384

99.422

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.091

97.977

0.697
  pilT Vibrio cholerae strain A1552

71.091

97.977

0.697
  pilT Neisseria gonorrhoeae MS11

69.501

98.555

0.685
  pilT Neisseria meningitidis 8013

69.501

98.555

0.685
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.711

0.514
  pilU Vibrio cholerae strain A1552

42.154

93.931

0.396
  pilU Pseudomonas stutzeri DSM 10701

40.181

95.665

0.384
  pilU Acinetobacter baylyi ADP1

38.838

94.509

0.367

Multiple sequence alignment