Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PS1M3_RS14030 Genome accession   NZ_AP022624
Coordinates   3003501..3004631 (-) Length   376 a.a.
NCBI ID   WP_203985029.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. PS1M3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2998501..3009631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS1M3_RS14000 (PS1M3_27350) - 2998842..2999735 (+) 894 WP_006793184.1 hypothetical protein -
  PS1M3_RS14005 (PS1M3_27360) - 2999809..3000285 (+) 477 WP_006793185.1 hypothetical protein -
  PS1M3_RS14010 (PS1M3_27370) rsmE 3000360..3001094 (+) 735 WP_008132394.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PS1M3_RS14015 (PS1M3_27380) gshB 3001171..3002121 (+) 951 WP_008132396.1 glutathione synthase -
  PS1M3_RS14020 (PS1M3_27390) - 3002292..3002849 (+) 558 WP_008132397.1 YqgE/AlgH family protein -
  PS1M3_RS14025 (PS1M3_27400) ruvX 3002978..3003427 (+) 450 WP_006793189.1 Holliday junction resolvase RuvX -
  PS1M3_RS14030 (PS1M3_27410) pilU 3003501..3004631 (-) 1131 WP_203985029.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PS1M3_RS14035 (PS1M3_27420) pilT 3004641..3005681 (-) 1041 WP_006793191.1 type IV pilus twitching motility protein PilT Machinery gene
  PS1M3_RS14040 (PS1M3_27430) - 3005684..3006397 (+) 714 WP_008132401.1 YggS family pyridoxal phosphate-dependent enzyme -
  PS1M3_RS14045 (PS1M3_27440) proC 3006415..3007236 (+) 822 WP_008132402.1 pyrroline-5-carboxylate reductase -
  PS1M3_RS14050 (PS1M3_27450) - 3007264..3007797 (+) 534 WP_006793194.1 YggT family protein -
  PS1M3_RS14055 (PS1M3_27460) - 3007857..3008303 (+) 447 WP_008132403.1 DUF4426 domain-containing protein -
  PS1M3_RS14060 (PS1M3_27470) - 3008308..3008907 (+) 600 WP_008132404.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41685.57 Da        Isoelectric Point: 5.6328

>NTDB_id=78974 PS1M3_RS14030 WP_203985029.1 3003501..3004631(-) (pilU) [Pseudoalteromonas sp. PS1M3]
MTLSLNRYLITMIEKKGSDLFVSSRLPVSAKINGELIPLGDDKLTDEESLQLIESAMSEKQKAEFHATKECNFAIATDEG
RFRISAFWQRDRAGMVIRRIVTQIPDVSDLGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLIPTSDGEGRVAAIEILLNSPMVAELIKNGDIGGIKEAMAKSNDMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGMGDDDN

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=78974 PS1M3_RS14030 WP_203985029.1 3003501..3004631(-) (pilU) [Pseudoalteromonas sp. PS1M3]
ATGACTTTATCTTTAAACCGTTACTTAATCACTATGATTGAGAAAAAAGGCTCTGATTTATTTGTATCTAGCCGTTTACC
TGTTAGTGCAAAAATAAACGGTGAATTAATTCCTTTGGGCGACGACAAACTCACTGACGAAGAGTCTTTACAGTTGATTG
AGTCTGCGATGAGCGAAAAGCAAAAAGCGGAGTTTCATGCTACTAAAGAATGTAACTTTGCTATTGCAACCGACGAAGGG
CGTTTTCGTATTTCTGCGTTTTGGCAGCGCGATAGAGCAGGTATGGTTATTCGCCGAATTGTGACACAGATCCCCGATGT
CAGTGATTTGGGCTTACCTTCTACACTTACCGACGTCATTATGGCTAAGCGCGGTTTAGTTCTGTTTGTTGGTGGAACAG
GAACCGGTAAGTCGACCTCATTAGCAGCCTTGATTGGCTACAGAAACCGAAACCAACGGGGTCACATTTTAACAATTGAA
GATCCTATTGAGTTTGTTCATGAGCACCGCAAAAGTATTATTACTCAACGCGAAGTAGGGCTCGATACAGATAGTTTTGA
GTCGGCGCTCAAAAGCTCATTACGTCAAGCTCCAGATGTTATTTTAATTGGTGAAATACGTTCGCAAGAAACTATGGAAT
ACGCATTAAGCTTTGCTGAAACAGGGCATTTATGTGTGGCTACACTACATGCCAACAATGCTAACCAAGCCATTGACCGT
ATTATGCATTTGGTACCAAAAGAGAAACATGACAAGCTAAAATACGATCTAGCCCTTAACTTACGTGCCATTGTTGCTCA
ACAATTAATACCTACCTCAGATGGCGAAGGACGCGTTGCCGCAATAGAGATATTACTTAATTCACCTATGGTGGCAGAGC
TGATTAAAAACGGCGATATTGGCGGCATTAAAGAGGCTATGGCTAAATCTAACGATATGGGCATGCAAACCTTCGACCAA
GCCCTTTTTGAGTTATACAGGCAGCAACGTATCAACTATGCAGATGCACTGCATCATGCTGACTCACCAAATGACTTACG
CTTGATGATTAAACTGCGCAATAACGAACAACAAGGTGCGGGCTTTTTACAAGGTGTAACTATTGATGGAATGGGGGATG
ACGATAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.56

100

0.577
  pilU Acinetobacter baylyi ADP1

56.499

100

0.566
  pilU Vibrio cholerae strain A1552

55.858

97.606

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.84

92.819

0.407

Multiple sequence alignment