Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUT72_RS12425 Genome accession   NZ_CP117994
Coordinates   2662186..2663409 (+) Length   407 a.a.
NCBI ID   WP_017635918.1    Uniprot ID   A0AAW9BMM9
Organism   Vibrio alginolyticus strain 20220413_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2657186..2668409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUT72_RS12405 (PUT72_12405) ampD 2658204..2658755 (-) 552 WP_025768186.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUT72_RS12410 (PUT72_12410) nadC 2658848..2659735 (+) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUT72_RS12415 (PUT72_12415) pilA 2659998..2660471 (+) 474 WP_065274839.1 pilin Machinery gene
  PUT72_RS12420 (PUT72_12420) pilB 2660477..2662162 (+) 1686 WP_225466547.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUT72_RS12425 (PUT72_12425) pilC 2662186..2663409 (+) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  PUT72_RS12430 (PUT72_12430) pilD 2663474..2664343 (+) 870 WP_017635919.1 prepilin peptidase Machinery gene
  PUT72_RS12435 (PUT72_12435) coaE 2664344..2664958 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  PUT72_RS12440 (PUT72_12440) zapD 2664986..2665726 (+) 741 WP_005373086.1 cell division protein ZapD -
  PUT72_RS12445 (PUT72_12445) yacG 2665885..2666079 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  PUT72_RS12450 (PUT72_12450) rplS 2666544..2666897 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  PUT72_RS12455 (PUT72_12455) trmD 2666939..2667700 (-) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PUT72_RS12460 (PUT72_12460) rimM 2667728..2668276 (-) 549 WP_005379964.1 ribosome maturation factor RimM -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45313.50 Da        Isoelectric Point: 10.3992

>NTDB_id=789031 PUT72_RS12425 WP_017635918.1 2662186..2663409(+) (pilC) [Vibrio alginolyticus strain 20220413_2]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=789031 PUT72_RS12425 WP_017635918.1 2662186..2663409(+) (pilC) [Vibrio alginolyticus strain 20220413_2]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCAGGCAAAAAGACATCTGGACAAAC
CCTCGCGATGACGGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAGCCATCGCGTGAAAGGCAGAGACATCACGGTGTTTACCCGTCAGATTTCGACGATGTTG
ATAACCGGTGTACCCTTAGTTCAGGCTTTAAAATTGGTCTCAGAAAATCATAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTCGAAGCGGGTACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGACCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCAGAAGTATTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCCCTGATTTACCCAGCCATGGTAGTGCTAGTAGCCTTAGGCGTCTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCTTGGTTTACCAGGC
AAGTATTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCATCTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCTGTTCTGGGCCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACACTATCAACTCGCCATAGAAGAAGTGTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTAGCCATGCGCAACTGTAATGTGTTCCCTGAATTGGTGTTACAAATGGTTATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGACAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTATTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

87.224

100

0.872
  pilC Vibrio cholerae strain A1552

73.775

100

0.74
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilC Legionella pneumophila strain ERS1305867

40.295

100

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398