Detailed information NTG0789032

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PUT72_RS12430
• Genome accession: NZ_CP117994
• Coordinates: 2663474..2664343 (+)
• Length: 289 a.a.
• NCBI ID: WP_017635919.1
• Uniprot ID: A0A2I3C9C8
• Organism: Vibrio alginolyticus strain 20220413_2
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2658474..2669343

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUT72_RS12410 (PUT72_12410)	nadC	2658848..2659735 (+)	888	WP_005379983.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PUT72_RS12415 (PUT72_12415)	pilA	2659998..2660471 (+)	474	WP_065274839.1	pilin	Machinery gene
PUT72_RS12420 (PUT72_12420)	pilB	2660477..2662162 (+)	1686	WP_225466547.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PUT72_RS12425 (PUT72_12425)	pilC	2662186..2663409 (+)	1224	WP_017635918.1	type II secretion system F family protein	Machinery gene
PUT72_RS12430 (PUT72_12430)	pilD	2663474..2664343 (+)	870	WP_017635919.1	prepilin peptidase	Machinery gene
PUT72_RS12435 (PUT72_12435)	coaE	2664344..2664958 (+)	615	WP_017635920.1	dephospho-CoA kinase	-
PUT72_RS12440 (PUT72_12440)	zapD	2664986..2665726 (+)	741	WP_005373086.1	cell division protein ZapD	-
PUT72_RS12445 (PUT72_12445)	yacG	2665885..2666079 (+)	195	WP_017635922.1	DNA gyrase inhibitor YacG	-
PUT72_RS12450 (PUT72_12450)	rplS	2666544..2666897 (-)	354	WP_005379971.1	50S ribosomal protein L19	-
PUT72_RS12455 (PUT72_12455)	trmD	2666939..2667700 (-)	762	WP_005385420.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
PUT72_RS12460 (PUT72_12460)	rimM	2667728..2668276 (-)	549	WP_005379964.1	ribosome maturation factor RimM	-
PUT72_RS12465 (PUT72_12465)	rpsP	2668306..2668554 (-)	249	WP_005379962.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32563.76 Da
• Isoelectric Point: 6.8450

>NTDB_id=789032 PUT72_RS12430 WP_017635919.1 2663474..2664343(+) (pilD) [Vibrio alginolyticus strain 20220413_2]
MEVFQYYPWLFVVFATIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIEPPKEVLTLSVPRSSCQKCATPIRIR
DNIPVISWLLLKGKCHNCQATISARYPLIELLTAFCSGFIAYYFGFSYFTVALLFFTFILIAATFIDLDTMLLPDQLTLP
LTWAGIALALVEISPVTLQDSVIGAIAGYLCLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGI
VFGIIQLRLQKQGIEKAFPFGPYLAIAGWISLIWGDQILNWYFTSVLGV

Nucleotide

• Length: 870 bp

>NTDB_id=789032 PUT72_RS12430 WP_017635919.1 2663474..2664343(+) (pilD) [Vibrio alginolyticus strain 20220413_2]
ATGGAAGTATTCCAGTACTACCCTTGGCTATTCGTTGTGTTTGCCACCATCTTCGGTCTGATCGTGGGTAGCTTCCTCAA
TGTGGTTATTTATCGACTACCTAAAATCATGGAACTAGAATGGCGACGCGAATGTGCCGAGTCATTTCCTGAATACAAAA
TAGAACCTCCTAAAGAAGTGCTGACCTTGAGCGTCCCACGCTCCTCCTGCCAGAAATGCGCAACACCGATACGTATTCGC
GACAACATTCCTGTGATTAGCTGGCTGCTTTTAAAAGGCAAATGTCACAACTGTCAGGCAACCATTAGTGCACGCTATCC
ACTGATTGAACTGCTCACCGCGTTCTGTTCAGGCTTTATAGCTTATTACTTTGGTTTTAGTTACTTTACCGTTGCCCTAC
TCTTTTTTACTTTTATCTTAATCGCCGCAACATTTATCGATCTCGATACTATGTTGTTACCTGACCAATTAACATTACCA
TTAACTTGGGCTGGCATTGCCTTAGCATTAGTCGAAATAAGTCCTGTTACTCTACAAGATTCTGTCATTGGTGCTATTGC
TGGTTACCTATGTCTCTGGTCCGTTTACTGGGGCTTTAAGTTACTTACCGGTAAAGAAGGAATGGGGTATGGTGATTTTA
AACTACTGGCGGCACTAGGAGCTTGGCTAGGTTGGCAGTCACTGCCGATGATCATTCTACTTTCTTCTGTGGTTGGCATC
GTATTTGGCATCATTCAACTACGCTTGCAAAAGCAGGGAATTGAAAAAGCTTTCCCTTTCGGTCCTTACTTAGCAATTGC
TGGTTGGATAAGCTTAATTTGGGGCGACCAGATTTTAAACTGGTACTTTACTTCAGTTCTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A2I3C9C8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	91.349	100	0.913
pilD	Vibrio cholerae strain A1552	73.264	99.654	0.73
pilD	Acinetobacter baumannii D1279779	52.222	93.426	0.488
pilD	Acinetobacter nosocomialis M2	51.852	93.426	0.484
pilD	Neisseria gonorrhoeae MS11	47.653	95.848	0.457