Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PUT72_RS12415 Genome accession   NZ_CP117994
Coordinates   2659998..2660471 (+) Length   157 a.a.
NCBI ID   WP_065274839.1    Uniprot ID   A0A7Y4EZC0
Organism   Vibrio alginolyticus strain 20220413_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2654998..2665471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUT72_RS12400 (PUT72_12400) pdhR 2657031..2657798 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PUT72_RS12405 (PUT72_12405) ampD 2658204..2658755 (-) 552 WP_025768186.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUT72_RS12410 (PUT72_12410) nadC 2658848..2659735 (+) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUT72_RS12415 (PUT72_12415) pilA 2659998..2660471 (+) 474 WP_065274839.1 pilin Machinery gene
  PUT72_RS12420 (PUT72_12420) pilB 2660477..2662162 (+) 1686 WP_225466547.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUT72_RS12425 (PUT72_12425) pilC 2662186..2663409 (+) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  PUT72_RS12430 (PUT72_12430) pilD 2663474..2664343 (+) 870 WP_017635919.1 prepilin peptidase Machinery gene
  PUT72_RS12435 (PUT72_12435) coaE 2664344..2664958 (+) 615 WP_017635920.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16576.11 Da        Isoelectric Point: 7.6275

>NTDB_id=789029 PUT72_RS12415 WP_065274839.1 2659998..2660471(+) (pilA) [Vibrio alginolyticus strain 20220413_2]
MKNSKQKKQQGFTLIELMIVVAIIGILSAFAVPAYQNYTKKATLSEFPKAAAAAKLAVELCAHENASNETTFISECVKEE
NGIPAKFVLNNIEISVVKATDASVSGAIAVIAAADEVKGPIAKGEQYIMAASYKTQGLEWGASCKAGGKAQTDYCPN

Nucleotide


Download         Length: 474 bp        

>NTDB_id=789029 PUT72_RS12415 WP_065274839.1 2659998..2660471(+) (pilA) [Vibrio alginolyticus strain 20220413_2]
ATGAAAAACAGTAAACAGAAAAAACAACAGGGTTTTACTCTGATTGAGTTAATGATTGTGGTGGCGATTATTGGCATCTT
ATCTGCTTTCGCAGTTCCTGCTTACCAAAATTATACTAAGAAAGCGACATTGTCAGAATTTCCAAAAGCAGCTGCGGCAG
CCAAATTAGCAGTAGAATTATGTGCCCATGAGAATGCCTCAAATGAAACAACATTTATTAGTGAATGTGTAAAGGAAGAA
AATGGAATTCCCGCTAAATTCGTTTTGAATAATATTGAAATTAGCGTTGTTAAAGCCACAGATGCTAGCGTTTCTGGTGC
GATAGCAGTTATTGCAGCAGCTGATGAAGTTAAGGGACCTATTGCTAAAGGCGAGCAATATATCATGGCTGCATCATATA
AAACTCAAGGCTTAGAGTGGGGGGCTTCATGTAAAGCTGGCGGTAAAGCACAAACAGACTACTGCCCAAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y4EZC0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.051

100

0.561
  pilA Haemophilus influenzae Rd KW20

42.361

91.72

0.389
  pilA Haemophilus influenzae 86-028NP

39.86

91.083

0.363